University of Palermo, Department of Oral Sciences G. Messina, Palermo, Italy.
Med Oral Patol Oral Cir Bucal. 2009 Nov 1;14(11):e558-62. doi: 10.4317/medoral.14.e558.
Oral Lichen Planus (OLP) is a chronic inflammatory disease with an autoimmune inflammatory pathogenesis. The aim of the research is to compare the vascular endothelial growth factor (VEGF) and adhesion of molecules in the biopsy samples of patients affected by OLP, in order to research the presence of the angiogenetic phenomenon and to understand its pathogenetic mechanism.
Thirty OLP patients and thirty healthy subjects were enrolled in a study. The immunohistochemical analysis of the VEGF and vascular-endothelial adhesion molecules was carried out by means of primary antibodies and anti-CD34, anti-VEGF, anti-CD106 antigen (VCAM-1) and anti-CD54 antigen (ICAM-1). The statistical significance of the differences was checked with the Mann-Whitney test (MW test). The level of significance was set to P<0.001. Data analysis was carried out with StatView 5.0.1 (SAS Institute Inc., Cary, NC).
The results reveal the presence of a significant angiogenesis in OLP patients for the VEGF, CD34, CD106 and CD54 (P < 0.001).. The number of vessels in the biopsies of the patients with OLP (mean +/-SD: 21.27+/-4.85), compared with the healthy subjects (mean +/-SD: 4.74+/-0.97) was significantly more (Mann-Whitney test, P < 0.001). The positive expression rate of VEGF, CD34, VCAM-1 and ICAM-1 in oral lichen samples was 64.2%, 54.3%, 32.5% and 29.7%, respectively. Isolated endothelial cells and newly-formed micro-vessels and endothelial cells with high-immune-positivity to the antibodies anti-ICAM-1 and anti-VCAM-1 were observed.
The results of our immunohistochemical research show that a significant neoangiogenesis occurs in oral lichen planus.
口腔扁平苔藓（OLP）是一种具有自身免疫炎症发病机制的慢性炎症性疾病。本研究旨在比较患有 OLP 的患者的活检样本中的血管内皮生长因子（VEGF）和黏附分子，以研究血管生成现象的存在并了解其发病机制。
共纳入 30 例 OLP 患者和 30 例健康受试者。采用针对 VEGF 和血管内皮黏附分子的一抗和抗 CD34、抗 VEGF、抗 CD106 抗原（VCAM-1）和抗 CD54 抗原（ICAM-1）进行免疫组织化学分析。采用 Mann-Whitney 检验（MW 检验）检查差异的统计学意义。显著性水平设定为 P<0.001。使用 StatView 5.0.1（SAS Institute Inc.，Cary，NC）进行数据分析。
结果显示，OLP 患者存在明显的血管生成，VEGF、CD34、CD106 和 CD54 均有显著差异（P<0.001）。与健康受试者（平均值 +/-标准差：4.74+/-0.97）相比，OLP 患者活检中的血管数量（平均值 +/-标准差：21.27+/-4.85）明显更多（Mann-Whitney 检验，P<0.001）。口腔扁平苔藓样本中 VEGF、CD34、VCAM-1 和 ICAM-1 的阳性表达率分别为 64.2%、54.3%、32.5%和 29.7%。观察到孤立的内皮细胞和新形成的微血管以及对抗体抗 ICAM-1 和抗 VCAM-1 具有高免疫阳性的内皮细胞。
我们的免疫组织化学研究结果表明，口腔扁平苔藓中存在显著的新生血管生成。
