Yadav N P, Chanda D, Chattopadhyay S K, Gupta A K, Pal A
Molecular Bioprospection, Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow - 226 015, India.
Indian J Pharm Sci. 2010 Nov;72(6):759-65. doi: 10.4103/0250-474X.84589.
The aim of the present work was to investigate the in vivo hepatoprotective potential of coumarinolignoids (cleomiscosins A, B, and C) isolated from the seeds of C. viscosa. The study was performed against CCl(4)-induced hepatotoxicity in albino rats. Rats were divided into four groups. The animals of group I served as normal and was given only vehicle. Group II served as toxin control and administered with CCl(4) (50% solution liquid paraffin, 2 ml/kg intraperitoneally). The animals of group III received coumarinolignoids (50 mg/kg) for six days orally as well as CCl(4) (2 ml/kg) on 4(th) day i.p. Similarly animals of group IV received silymarin (50 mg/kg) for six days orally as well as CCl(4) on 4(th) day i.p. On 7(th) day various parameters viz. serum glutamyl oxaloacetic transaminase, serum glutamyl pyruvate transaminase, serum alkaline phosphatase, serum bilirubin, liver glycogen were estimated and histopathology was performed. Additionally, acute oral toxicity of the said coumarinolignoids was carried out in swiss albino mice. The coumarinolignoids were found to be effective as hepatoprotective against CCl(4)-induced hepatotoxicity as evidenced by in vivo and histopathological studies in small animals. Safety evaluation studies also exhibit that coumarinolignoids are well tolerated by small animals in acute oral toxicity study except minor changes in red blood cell count and hepatic protein content at 5000 mg/kg body weight as a single oral dose. Coumarinolignoids which is the mixture of three compounds (cleomiscosin A, B and C) is showing the significant protective effects against CCl(4)-induced hepatotoxicity in small animals and also coumarinolignoids are well tolerated by small animals in acute oral study.
本研究的目的是调查从粘毛婆罗门参种子中分离得到的香豆素木脂素（臭矢菜素A、B和C）的体内肝脏保护潜力。该研究针对四氯化碳诱导的白化大鼠肝毒性进行。大鼠被分为四组。第一组动物作为正常对照组，仅给予赋形剂。第二组作为毒素对照组，腹腔注射四氯化碳（50%溶液的液体石蜡，2 ml/kg）。第三组动物口服香豆素木脂素（50 mg/kg），持续六天，并在第4天腹腔注射四氯化碳（2 ml/kg）。同样，第四组动物口服水飞蓟宾（50 mg/kg），持续六天，并在第4天腹腔注射四氯化碳。在第7天，测定了各种参数，即血清谷草转氨酶、血清谷丙转氨酶、血清碱性磷酸酶、血清胆红素、肝糖原，并进行了组织病理学检查。此外，还对白化瑞士小鼠进行了上述香豆素木脂素的急性口服毒性试验。体内和小动物组织病理学研究表明，香豆素木脂素对四氯化碳诱导的肝毒性具有有效的肝脏保护作用。安全性评估研究还表明，在急性口服毒性试验中，除了单次口服剂量为5000 mg/kg体重时红细胞计数和肝脏蛋白质含量有轻微变化外，小动物对香豆素木脂素耐受性良好。由三种化合物（臭矢菜素A、B和C）组成的香豆素木脂素对小动物四氯化碳诱导的肝毒性显示出显著的保护作用，并且在急性口服研究中，小动物对香豆素木脂素耐受性良好。
