de la Peña Salvador Sánchez, Sothern Robert B, López Fernando Santillán, Lujambio Irene Mendoza, Waizel-Bucay José, Sánchez Carolina Olarte, Monroy Claudia Pérez, Betancourt Eduardo Tena
Chronomics Research Center at Sección de Estudios de Posgrado e Investigación (SEPI)-Escuela Nacional de Medicina y Homeopatía (ENMyH), Instituto Politécnico Nacional (IPN).
Pharmacogn Mag. 2011 Jul;7(27):234-42. doi: 10.4103/0973-1296.84238.
Aconitum napellus (Acn) is used topically to relieve pain, itching and inflammation, and internally to reduce febrile states, among others. Any circadian time-related consequences of Acn administration are unknown. The objective of this study was to explore the effects of two doses of Acn on body temperature (BT) of mice treated at six different times over 24 hours.
BALB/c female mice were housed in six chambers (six mice each) with air temperature 24 ± 3°C, humidity 60 ± 4%, and a 12-hours light (L)/12-hours dark cycle, but with L-onset staggered by 4 hours between chambers so that study at one external test time resulted in six test times (02, 06, 10, 14, 18 and 22 hours [h] after light onset). Rectal temperature (RT; in °C) was measured at baseline (B) and 1 hour after oral treatment with placebo (P) or two doses of Acn (6C and 30C, two studies each) in six studies over an 8 day span. The difference in RT for each mouse from the respective B + P timepoint mean RT was computed following each Acn treatment, and data from each of the six studies (original RT and difference from B + P) were analyzed for time-effect by analysis of variance (ANOVA) and for circadian rhythm by 24-hour cosine fitting.
A CIRCADIAN RHYTHM IN RT WAS FOUND AT B AND AFTER P (MEAN: 35.58°C vs. 35.69°C; peak: 15:31 h vs. 15:40 h) and after each Acn dose (30C or 6C). Acn induced hyperthermia and the overall change in BT was rhythmically significant for each dose (mean = +1.95°C vs. +1.70°C), with greatest hyperthermia observed during the L-span for each dose (peak = 08:56 h vs. 05:17 h).
Acn administered around the clock induced hyperthermia overall and in a time-dependent manner, with greatest effects during the resting (L) span. Thus, time of day may significantly impact the outcome of Acn and other homeopathic treatments and should be considered in determining optimal dosing and treatment time(s) in order to increase the desired outcome and decrease undesired effects.
欧乌头外用可缓解疼痛、瘙痒和炎症，内服可降低发热状态等。欧乌头给药的任何与昼夜节律时间相关的后果尚不清楚。本研究的目的是探讨两剂欧乌头对在24小时内六个不同时间点接受治疗的小鼠体温（BT）的影响。
将BALB/c雌性小鼠饲养在六个实验舱中（每个实验舱六只），舱内气温为24±3°C，湿度为60±4%，光照/黑暗周期为12小时，各实验舱之间光照开始时间错开4小时，以便在一个外部测试时间进行的研究产生六个测试时间点（光照开始后02、06、10、14、18和22小时）。在8天内的六项研究中，于基线（B）以及口服安慰剂（P）或两剂欧乌头（6C和30C，每项研究各两剂）后1小时测量直肠温度（RT；单位为°C）。每次欧乌头治疗后，计算每只小鼠相对于各自B+P时间点平均RT的RT差值，并通过方差分析（ANOVA）对六项研究中每项研究的数据（原始RT以及与B+P的差值）进行时间效应分析，通过24小时余弦拟合进行昼夜节律分析。
在基线时以及服用安慰剂后（平均值：35.58°C对35.69°C；峰值：15:31小时对15:40小时）以及每次服用欧乌头剂量（30C或6C）后，均发现直肠温度存在昼夜节律。欧乌头引起体温过高，且每种剂量的体温总体变化在节律上具有显著性（平均值=+1.95°C对+1.70°C），每种剂量在光照期间观察到最大体温过高（峰值=08:56小时对05:17小时）。
全天给药的欧乌头总体上以时间依赖性方式引起体温过高，在休息（光照）期间影响最大。因此，一天中的时间可能会显著影响欧乌头和其他顺势疗法治疗的结果，在确定最佳给药剂量和治疗时间时应予以考虑，以增加预期疗效并减少不良影响。
