Stenman G, Kindblom L G, Willems J, Angervall L
Department of Oral Pathology, Gothenburg University, Sweden.
Cancer. 1990 May 1;65(9):2006-13. doi: 10.1002/1097-0142(19900501)65:9<2006::aid-cncr2820650921>3.0.co;2-q.
The chromosomal banding pattern and the in vitro growth characteristics of a metastatic epithelioid sarcoma are described. The cultured tumor cells retained growth characteristics as well as ultrastructural and immunohistochemical properties similar to the cells of the primary tumor. Cytogenetic analysis revealed a modal range in the diploid-hypodiploid region, a finding which was corroborated by quantitative DNA determinations of both the primary tumor and a lymph node metastasis. Fourteen different marker chromosomes were identified. The most frequent clonal rearrangement was a 1p-marker resulting from a short arm terminal deletion, i.e., del (1) (p21-22). A similar 1p- marker has previously been observed in an established epithelioid sarcoma cell line. The finding of an apparently identical 1p-marker in two of two analyzed epithelioid sarcomas suggests that this rearrangement may be a primary cytogenetic abnormality in epithelioid sarcoma. An elevated ras p21 expression was demonstrated using immunohistochemical methods. The possible involvement of the N-ras gene and/or a tumor suppressor in the 1p deletion is considered.
本文描述了转移性上皮样肉瘤的染色体带型模式及体外生长特征。培养的肿瘤细胞保留了与原发肿瘤细胞相似的生长特征、超微结构和免疫组化特性。细胞遗传学分析显示二倍体 - 亚二倍体区域存在一个模式范围，这一发现通过对原发肿瘤和淋巴结转移灶的定量DNA测定得到了证实。共鉴定出14条不同的标记染色体。最常见的克隆重排是由短臂末端缺失导致的1号染色体短臂标记，即del(1)(p21 - 22)。此前在一个已建立的上皮样肉瘤细胞系中也观察到了类似的1号染色体短臂标记。在两个分析的上皮样肉瘤中，有两个出现了明显相同的1号染色体短臂标记，这表明这种重排可能是上皮样肉瘤的原发性细胞遗传学异常。使用免疫组化方法证实了ras p21表达升高。本文还考虑了N - ras基因和/或肿瘤抑制因子可能参与1号染色体短臂缺失的情况。
