Becher R, Wake N, Gibas Z, Ochi H, Sandberg A A
J Natl Cancer Inst. 1984 Apr;72(4):823-31.
An analysis of chromosome aberrations in human tumors was performed in 29 cases of soft tissue sarcoma. The tumor tissues were disaggregated with collagenase and the cells cultured for 2-3 days. Analyzable metaphases were obtained in 15 cases, 4 of which showed only normal karyotypes. The remaining 11 tumors showed various numerical and structural abnormalities in their karyotypes: 8 tumors were near-diploid and the remaining 3 were near-triploid. G- and Q-banding analyses revealed clonal abnormalities in the 11 cases with the presence of marker chromosomes; 15 different chromosomes were involved in chromosome rearrangements, chromosomes 1 and 2 being the most frequently affected. Because of the heterogeneity of the tumor group investigated (neurogenic sarcoma, 2 liposarcomas, neurofibrosarcoma, synovial cell sarcoma, fibrosarcoma, mesothelioma, leiomyosarcoma, rhabdomyosarcoma, Ewing's sarcoma, and hemangiopericytoma), it was impossible to reach any conclusion on the specificity of the cytogenetic abnormalities for a particular tumor type.
对29例软组织肉瘤患者的肿瘤细胞进行了染色体畸变分析。用胶原酶将肿瘤组织解离，细胞培养2 - 3天。15例获得了可分析的中期分裂相，其中4例仅显示正常核型。其余11个肿瘤的核型显示出各种数目和结构异常：8个肿瘤接近二倍体，其余3个接近三倍体。G带和Q带分析显示，11例存在标记染色体的病例有克隆异常；15条不同的染色体参与了染色体重排，其中1号和2号染色体受影响最为频繁。由于所研究的肿瘤组具有异质性（包括神经源性肉瘤、2例脂肪肉瘤、神经纤维肉瘤、滑膜细胞肉瘤、纤维肉瘤、间皮瘤、平滑肌肉瘤、横纹肌肉瘤、尤因肉瘤和血管外皮细胞瘤），因此无法就特定肿瘤类型的细胞遗传学异常特异性得出任何结论。
