实时 PCR 法检测生活在法国的神经囊虫病患者脑脊液样本中的囊虫。
Confirmation and follow-up of neurocysticercosis by real-time PCR in cerebrospinal fluid samples of patients living in France.
机构信息
Laboratoire de Parasitologie Mycologie, Hôpital Cochin-Port Royal, AP-HP, Université Paris Descartes, 27 rue du Faubourg Saint-Jacques, 75014 Paris, France.
出版信息
J Clin Microbiol. 2011 Dec;49(12):4338-40. doi: 10.1128/JCM.05839-11. Epub 2011 Oct 5.
Abstract
Neurocysticercosis diagnosis is based on a combination of clinical, epidemiological, radiological, and immunological findings. We describe a real-time PCR assay for the confirmation of neurocysticercosis diagnosis in cerebrospinal fluid. The assay, tested on samples from nine patients living in France and diagnosed with neurocysticercosis, had a detection rate of 83.3% and 100% specificity.
摘要
神经囊虫病的诊断基于临床、流行病学、影像学和免疫学检查的综合结果。我们描述了一种实时 PCR 检测方法，用于确认脑脊液中的神经囊虫病诊断。该检测方法在 9 名居住在法国并被诊断为神经囊虫病的患者样本中进行了测试，其检测率为 83.3%，特异性为 100%。
相似文献
1
Confirmation and follow-up of neurocysticercosis by real-time PCR in cerebrospinal fluid samples of patients living in France.实时 PCR 法检测生活在法国的神经囊虫病患者脑脊液样本中的囊虫。
J Clin Microbiol. 2011 Dec;49(12):4338-40. doi: 10.1128/JCM.05839-11. Epub 2011 Oct 5.
2
Human neurocysticercosis: comparison of different diagnostic tests using cerebrospinal fluid.人脑囊虫病：脑脊液不同诊断检测方法的比较。
J Clin Microbiol. 2011 Jan;49(1):195-200. doi: 10.1128/JCM.01554-10. Epub 2010 Nov 10.
3
HDP2: a ribosomal DNA (NTS-ETS) sequence as a target for species-specific molecular diagnosis of intestinal taeniasis in humans.HDP2：核糖体 DNA（NTS-ETS）序列作为人类肠道带绦虫病种特异性分子诊断的靶标。
Parasit Vectors. 2018 Feb 27;11(1):117. doi: 10.1186/s13071-018-2646-6.
4
Jacalin-unbound fraction of Taenia saginata in immunodiagnosis of neurocysticercosis in human cerebrospinal fluid.猪带绦虫 Jacalin 结合物在人脑脊液中囊尾蚴病免疫诊断中的非结合部分。
Diagn Microbiol Infect Dis. 2010 Nov;68(3):259-64. doi: 10.1016/j.diagmicrobio.2010.06.016. Epub 2010 Sep 16.
5
First Case of Human Cerebral Taenia martis Cysticercosis.人类脑型马绦虫囊尾蚴病首例病例。
J Clin Microbiol. 2015 Aug;53(8):2756-9. doi: 10.1128/JCM.01033-15. Epub 2015 May 27.
6
Isolation of the Taenia crassiceps antigens from a phage display cDNA library and evaluation of their use for diagnosis of neurocysticercosis.从噬菌体展示cDNA文库中分离肥胖带绦虫抗原及其用于神经囊尾蚴病诊断的评估。
Clin Immunol. 2005 Sep;116(3):265-70. doi: 10.1016/j.clim.2005.04.009.
7
Cysticercus antigens in cerebrospinal fluid samples from patients with neurocysticercosis.来自神经囊尾蚴病患者脑脊液样本中的囊尾蚴抗原。
J Clin Microbiol. 2001 Sep;39(9):3368-72. doi: 10.1128/JCM.39.9.3368-3372.2001.
8
Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for immunodiagnosis of neurocysticercosis using ELISA on cerebrospinal fluid samples.利用酶联免疫吸附测定法（ELISA）检测脑脊液样本，评估猪带绦虫和肥胖带绦虫囊尾蚴抗原用于神经囊尾蚴病免疫诊断的效果。
Rev Soc Bras Med Trop. 2007 Mar-Apr;40(2):152-5. doi: 10.1590/s0037-86822007000200002.
9
Comparison of a new multiplex real-time PCR with the Kato Katz thick smear and copro-antigen ELISA for the detection and differentiation of Taenia spp. in human stools.一种新型多重实时聚合酶链反应与加藤厚涂片法及粪便抗原酶联免疫吸附测定法在检测和鉴别人体粪便中带绦虫属的比较
PLoS Negl Trop Dis. 2017 Jul 7;11(7):e0005743. doi: 10.1371/journal.pntd.0005743. eCollection 2017 Jul.
10
Neurocysticercosis: detection of Taenia solium DNA in human cerebrospinal fluid using a semi-nested PCR based on HDP2.神经囊尾蚴病：基于HDP2的半巢式PCR检测人脑脊液中猪带绦虫DNA
Ann Trop Med Parasitol. 2008 Jun;102(4):317-23. doi: 10.1179/136485908X278856.
引用本文的文献
1
Molecular prevalence of in sheep exhibiting neurological symptoms in Kazakhstan.哈萨克斯坦出现神经症状的绵羊中的分子流行情况 。 （你提供的原文“Molecular prevalence of in sheep exhibiting neurological symptoms in Kazakhstan.”中“Molecular prevalence of ”后面似乎缺少具体内容）
Front Vet Sci. 2025 Jul 1;12:1620425. doi: 10.3389/fvets.2025.1620425. eCollection 2025.
2
Concomitant Parenchymal, Subarachnoid, and Ventricular Neurocysticercosis in Rome, Italy: A Case Report with a 4-Year Follow-Up.意大利罗马的脑实质、蛛网膜下腔和脑室同时发生的神经囊尾蚴病：一例随访4年的病例报告
Trop Med Infect Dis. 2024 Aug 21;9(8):187. doi: 10.3390/tropicalmed9080187.
3
Neurocysticercosis Diagnosis in a Non-Endemic Country: France.非流行国家（法国）的神经囊尾蚴病诊断
Pathogens. 2023 Sep 29;12(10):1205. doi: 10.3390/pathogens12101205.
4
Next-generation sequencing of cerebrospinal fluid for the diagnosis and monitoring of neurocysticercosis.用于神经囊尾蚴病诊断和监测的脑脊液下一代测序
Acta Neurol Belg. 2024 Apr;124(2):647-650. doi: 10.1007/s13760-023-02345-4. Epub 2023 Aug 9.
5
Next-generation sequencing combined with serological tests based pathogen analysis for a neurocysticercosis patient with a 20-year history:a case report.基于病原体分析的新一代测序联合血清学检测用于一位有 20 年病史的神经囊虫病患者：病例报告。
BMC Neurol. 2021 Jun 24;21(1):236. doi: 10.1186/s12883-021-02277-7.
6
Collaborative Studies for the Detection of spp. Infections in Humans within CYSTINET, the European Network on Taeniosis/Cysticercosis.欧洲猪带绦虫病/囊尾蚴病网络CYSTINET内关于检测人体 spp. 感染的合作研究。
Microorganisms. 2021 May 29;9(6):1173. doi: 10.3390/microorganisms9061173.
7
Neurocysticercosis Diagnosed by PCR on Brain Biopsy.通过脑活检聚合酶链反应诊断神经囊尾蚴病
Case Rep Infect Dis. 2020 Nov 20;2020:8887647. doi: 10.1155/2020/8887647. eCollection 2020.
8
Subarachnoid neurocysticercosis: emerging concepts and treatment.蛛网膜下腔神经囊尾蚴病：新出现的概念和治疗方法。
Curr Opin Infect Dis. 2020 Oct;33(5):339-346. doi: 10.1097/QCO.0000000000000669.
9
Taenia solium Cysticercosis and Its Impact in Neurological Disease.猪带绦虫囊尾蚴病及其在神经疾病中的影响。
Clin Microbiol Rev. 2020 May 27;33(3). doi: 10.1128/CMR.00085-19. Print 2020 Jun 17.
10
Prevalence of, and Factors Associated with Intestinal Parasites in Multinational Expatriate Workers in Al Ain City, United Arab Emirates: An Occupational Cross-Sectional Study.在阿拉伯联合酋长国艾因市的多国侨民工人中，肠道寄生虫的流行情况及其相关因素：一项职业横断面研究。
J Immigr Minor Health. 2020 Apr;22(2):359-374. doi: 10.1007/s10903-019-00903-8.
本文引用的文献
1
Human neurocysticercosis: comparison of different diagnostic tests using cerebrospinal fluid.人脑囊虫病：脑脊液不同诊断检测方法的比较。
J Clin Microbiol. 2011 Jan;49(1):195-200. doi: 10.1128/JCM.01554-10. Epub 2010 Nov 10.
2
Taenia solium DNA is present in the cerebrospinal fluid of neurocysticercosis patients and can be used for diagnosis.猪带绦虫DNA存在于神经囊尾蚴病患者的脑脊液中，可用于诊断。
Eur Arch Psychiatry Clin Neurosci. 2006 Aug;256(5):307-10. doi: 10.1007/s00406-006-0612-3. Epub 2006 Jul 10.
3
[Cysticercosis contracted in metropolitan France].[在法国大都市感染的囊尾蚴病]
Presse Med. 2006 Feb;35(2 Pt 1):243-5. doi: 10.1016/s0755-4982(06)74562-6.
4
Putative Diphyllobothrium nihonkaiense acquired from a Pacific salmon (Oncorhynchus keta) eaten in France; genomic identification and case report.从在法国食用的太平洋鲑鱼（大麻哈鱼）中获得的疑似日本裂头绦虫；基因组鉴定及病例报告。
Parasitol Int. 2006 Mar;55(1):45-9. doi: 10.1016/j.parint.2005.09.004. Epub 2005 Oct 20.
5
Intraventricular neurocysticercosis: 18 consecutive patients and review of the literature.脑室内神经囊尾蚴病：18例连续病例及文献复习
Neurosurg Focus. 2002 Jun 15;12(6):e5. doi: 10.3171/foc.2002.12.6.6.
6
Neurocysticercosis: a review.神经囊尾蚴病：综述
Surg Neurol. 2005 Feb;63(2):123-32; discussion 132. doi: 10.1016/j.surneu.2004.02.033.
7
An unusual cause of psychosis.一种不寻常的精神病病因。
Lancet. 2004 May 8;363(9420):1522. doi: 10.1016/S0140-6736(04)16152-7.
8
Tapeworms and seizures--treatment and prevention.绦虫与癫痫——治疗与预防
N Engl J Med. 2004 Jan 15;350(3):215-7. doi: 10.1056/NEJMp038204.
9
Proposed diagnostic criteria for neurocysticercosis.神经囊尾蚴病的拟诊标准。
Neurology. 2001 Jul 24;57(2):177-83. doi: 10.1212/wnl.57.2.177.
10
Isolation and characterization of species-specific DNA probes from Taenia solium and Taenia saginata and their use in an egg detection assay.从猪带绦虫和牛带绦虫中分离并鉴定物种特异性DNA探针及其在虫卵检测试验中的应用。
J Clin Microbiol. 1995 May;33(5):1283-8. doi: 10.1128/jcm.33.5.1283-1288.1995.
Zotero 插件