Rudrakshi C, Srinivas N, Mehta D S
Department of Periodontology and Implantology, Bapuji Dental College and Hospital, Davangere, Karnataka, India.
J Indian Soc Periodontol. 2011 Apr;15(2):147-51. doi: 10.4103/0972-124X.84384.
The purpose of the present study was to evaluate and compare the relationship between the levels of hepatocyte growth factor (HGF) in gingival crevicular fluid (GCF) and saliva, both clinically and biochemically, in patients with and without chronic periodontitis.
For this study, 36 patients (12 male, 24 female) were selected in the age range of 18-60 years (mean, 32.7±11.1 years). The subjects were assigned to three groups, which included Group I (control), Group II (chronic periodontitis with probing depth PD <5 mm), and Group III (chronic periodontitis with PD ≥5 mm). Clinical parameters included plaque index, gingival index, sulcus bleeding index, PD, and clinical attachment level. The GCF samples were taken by using the capillary tubes whereas saliva was collected by the suction method. The levels of HGF in GCF and in saliva were estimated using an enzyme linked immunosorbant assay reader.
There was a significant correlation in the levels of HGF in GCF and in saliva of patients with and without chronic periodontitis. The results also indicated that the HGF levels in GCF and saliva correlated well with the clinical parameters and with the severity of the periodontal disease.
Both GCF and saliva can be used to estimate the levels of HGF and thus may be regarded as a novel marker for periodontal disease activity.
本研究旨在从临床和生化角度评估和比较慢性牙周炎患者与非慢性牙周炎患者龈沟液（GCF）和唾液中肝细胞生长因子（HGF）水平之间的关系。
本研究选取了36例年龄在18至60岁之间（平均年龄32.7±11.1岁）的患者（男性12例，女性24例）。受试者被分为三组，包括第一组（对照组）、第二组（探诊深度PD<5mm的慢性牙周炎患者）和第三组（PD≥5mm的慢性牙周炎患者）。临床参数包括菌斑指数、牙龈指数、龈沟出血指数、PD和临床附着水平。使用毛细管采集GCF样本，通过抽吸法收集唾液。使用酶联免疫吸附测定仪评估GCF和唾液中HGF的水平。
慢性牙周炎患者与非慢性牙周炎患者的GCF和唾液中HGF水平存在显著相关性。结果还表明，GCF和唾液中的HGF水平与临床参数以及牙周疾病的严重程度密切相关。
GCF和唾液均可用于评估HGF水平，因此可被视为牙周疾病活动的新型标志物。
