Sabbah A, Langlois P
Laboratoire d'Immuno-Allergologie, CHRU, Angers.
Allerg Immunol (Paris). 1990 May;22(5):173-8.
Every improvement can make the measurement of specific IgE more effective. This is the case with the CAP system, a new solid phase technique which uses an Immunocap made from hydrophilic polymer, encased in a capsule, for the fixation of anti-IgE or allergen. The Immunocap fixes 3 times more protein than paper discs. Another improvement is that of calibration of the CAP System by the WHO standard for IgE, with the results expressed in KUI/I: 0.35 to 100 KUI/I for specific IgE and 2 to 2,000 KUI/I for total IgE. The study was of patients who had an anaphylactic or systemic reaction after wasp stings. The subjects were divided into two groups A and B and compared with a reference Groupe C. The study also included subjects who were sensitive to mites, grass pollens and egg. Finally, the technique itself was submitted to a reproducibility test. The results confirm that the sensitivity and specificity of the test were both equal to unity. The CAP System therefore offers the possibility of detecting the lowest amounts of IgE.
每一项改进都能使特异性IgE的检测更有效。CAP系统就是这样，它是一种新的固相技术，使用由亲水性聚合物制成、封装在胶囊中的免疫帽来固定抗IgE或过敏原。免疫帽固定的蛋白量是纸盘的3倍。另一项改进是采用世界卫生组织IgE标准对CAP系统进行校准，结果以KUI/I表示：特异性IgE为0.35至100 KUI/I，总IgE为2至2000 KUI/I。该研究针对的是被黄蜂蜇伤后出现过敏或全身反应的患者。研究对象被分为A组和B组，并与参照C组进行比较。该研究还纳入了对螨虫、草花粉和鸡蛋过敏的对象。最后，对该技术本身进行了重复性测试。结果证实该检测的灵敏度和特异性均等于1。因此，CAP系统能够检测出最低含量的IgE。
