Elshazly Atef M, Attia Gehan, El-Ghareeb Azza S A, Belal Usama S
Department of Parasitology, Faculty of Medicine, University of Mansoura, Egypt.
J Egypt Soc Parasitol. 2011 Aug;41(2):263-74.
Human toxocariasis is a worldwide parasitic disease. Children are more frequently infected because of the closer contact with contaminated soil and relatively frequent geophagia. Toxocariasis in children has variable modes of presentation but clinical diagnosis is difficult. Various clinical phenotypes of toxocariasis in symptomatic children attending Children's Hospital Mansoura University were studied. A total of 480 children were included in the study with mean age 7.24 +/- 4.22 years, 61.9% were boys and 200 age-sex-matched healthy controls. Patients were examined clinically, and the anti-Toxocara antibodies in the blood of children were performed by ELISA using T. canis larval excretory-secretory products as antigen. Eosinophils level in peripheral blood was measured. Sero-positive cases were 12 % of patients and only 3.5% of controls. Statistical analysis showed a significant association between infection and male sex (P <0.001). Sero-positive children were older than the sero-negative (P <0.001). Eosinophilia was detected in 86.2% of sero-positive children. Sero-positivity and degree of eosinophilia were more frequently detected among patients with allergy (bronchial asthma and urticaria). Degree of eosinophilia was found to be positively correlated to the optical density (OD) ELISA of anti-Toxocara IgG.
人类弓蛔虫病是一种全球性的寄生虫病。儿童由于与受污染土壤接触更密切且异食癖相对频繁，因而感染更为常见。儿童弓蛔虫病的表现形式多样，但临床诊断困难。本研究对曼苏拉大学儿童医院有症状儿童弓蛔虫病的各种临床表型进行了研究。共有480名儿童纳入研究，平均年龄7.24±4.22岁，其中61.9%为男孩，并选取了200名年龄和性别匹配的健康对照。对患者进行临床检查，以犬弓蛔虫幼虫排泄分泌产物为抗原，采用酶联免疫吸附测定法（ELISA）检测儿童血液中的抗弓蛔虫抗体。测定外周血嗜酸性粒细胞水平。血清阳性病例占患者的12%，而对照中仅占3.5%。统计分析显示感染与男性性别之间存在显著关联（P<0.001）。血清阳性儿童比血清阴性儿童年龄更大（P<0.001）。86.2%的血清阳性儿童检测到嗜酸性粒细胞增多。在患有过敏（支气管哮喘和荨麻疹）的患者中，血清阳性和嗜酸性粒细胞增多程度更为常见。发现嗜酸性粒细胞增多程度与抗弓蛔虫IgG的酶联免疫吸附测定光密度（OD）呈正相关。
