Department of Clinical Analysis and Toxicology, UFRN, Natal, RN, Brazil.
Appl Physiol Nutr Metab. 2011 Oct;36(5):693-7. doi: 10.1139/h11-074. Epub 2011 Oct 7.
The generation of reactive oxygen species resulting from physical activity may trigger adaptive processes at the reproductive level and in the antioxidant defense system itself. The objective of this study was to investigate the effects of moderate daily swimming and lipoic acid (LA) supplementation on estrous cycle duration and pro-oxident and antioxidant markers in young Wistar rats. Animals were submitted to daily swimming (for 1 h) for 30 days, between 1300 h and 1400 h. The following study groups were formed: group 1, sedentary; group 2, submitted to swimming; group 3, sedentary supplemented with 100 mg·kg(-1)·day(-1) of LA; and group 4, submitted to swimming and supplementation with 100 mg·kg(-1)·day(-1) of LA. The estrous cycle of the animals was evaluated daily, and the following oxidative stress markers were measured: plasma thiobarbituric acid reactive substances (TBARS) and glutathione (GSH), erythrocyte superoxide dismutase (SOD), GSH peroxidase (GPx) and catalase (CAT) activity. The exercise protocol increased estrous cycle duration in group 2, especially in the diestrous phase. There was also a decrease in lipoperoxidation, with enhanced antioxidant activity of SOD and GPx. Group 4 showed no alteration in estrous cycle duration and maintained the beneficial effects on the antioxidant system observed in group 2. The increase in estrous cycle duration and improved oxidative stress markers may be an adaptive response to moderate exercise. LA impeded any exercise-induced alteration in the cycle but preserved improvements in the antioxidant system.
运动产生的活性氧可能会在生殖水平和抗氧化防御系统本身引发适应性过程。本研究的目的是研究适度的每日游泳和硫辛酸（LA）补充对年轻 Wistar 大鼠发情周期持续时间和前氧化剂和抗氧化标志物的影响。动物每天游泳（1 小时）30 天，时间在 1300 小时到 1400 小时之间。形成了以下研究组：第 1 组，久坐不动；第 2 组，游泳；第 3 组，久坐不动，每天补充 100mg·kg(-1)·day(-1)LA；第 4 组，游泳，每天补充 100mg·kg(-1)·day(-1)LA。每天评估动物的发情周期，并测量以下氧化应激标志物：血浆硫代巴比妥酸反应物质（TBARS）和谷胱甘肽（GSH）、红细胞超氧化物歧化酶（SOD）、GSH 过氧化物酶（GPx）和过氧化氢酶（CAT）活性。运动方案增加了第 2 组发情周期的持续时间，特别是在间情期。脂质过氧化作用也减少了，SOD 和 GPx 的抗氧化活性增强。第 4 组发情周期持续时间没有变化，并保持了第 2 组观察到的对抗氧化系统的有益影响。发情周期持续时间的增加和氧化应激标志物的改善可能是对适度运动的适应性反应。LA 阻止了运动引起的周期变化，但保持了抗氧化系统的改善。
