Laboratory of Nutrition and Metabolism, Faculty of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil.
Amino Acids. 2012 Aug;43(2):709-15. doi: 10.1007/s00726-011-1121-x. Epub 2011 Oct 19.
The objective of this study was to evaluate the effect of creatine supplementation on muscle and plasma markers of oxidative stress after acute aerobic exercise. A total of 64 Wistar rats were divided into two groups: control group (n = 32) and creatine-supplemented group (n = 32). Creatine supplementation consisted of the addition of 2% creatine monohydrate to the diet. After 28 days, the rats performed an acute moderate aerobic exercise bout (1-h swimming with 4% of total body weight load). The animals were killed before (pre) and at 0, 2 and 6 h (n = 8) after acute exercise. As expected, plasma and total muscle creatine concentrations were significantly higher (P < 0.05) in the creatine-supplemented group compared to control. Acute exercise increased plasma thiobarbituric acid reactive species (TBARS) and total lipid hydroperoxide. The same was observed in the soleus and gastrocnemius muscles. Creatine supplementation decreased these markers in plasma (TBARS: pre 6%, 0 h 25%, 2 h 27% and 6 h 20%; plasma total lipid hydroperoxide: pre 38%, 0 h 24%, 2 h 12% and 6 h 20%, % decrease). Also, acute exercise decreased the GSH/GSSG ratio in soleus muscle, which was prevented by creatine supplementation (soleus: pre 8%, 0 h 29%, 2 h 30% and 6 h 44%, % prevention). The results show that creatine supplementation inhibits increased oxidative stress markers in plasma and muscle induced by acute exercise.
本研究旨在评估补充肌酸对急性有氧运动后肌肉和血浆氧化应激标志物的影响。共有 64 只 Wistar 大鼠分为两组：对照组（n = 32）和肌酸补充组（n = 32）。肌酸补充组在饮食中添加 2%肌酸一水合物。28 天后，大鼠进行了一次急性中度有氧运动（1 小时游泳，负重 4%体重）。动物在急性运动前（pre）和 0、2 和 6 小时（n = 8）时被处死。正如预期的那样，与对照组相比，肌酸补充组的血浆和总肌肉肌酸浓度显著升高（P < 0.05）。急性运动增加了血浆硫代巴比妥酸反应物质（TBARS）和总脂质过氧化物。在比目鱼肌和腓肠肌中也观察到了同样的情况。肌酸补充降低了血浆中的这些标志物（TBARS：pre 6%，0 h 25%，2 h 27%和 6 h 20%；血浆总脂质过氧化物：pre 38%，0 h 24%，2 h 12%和 6 h 20%，%减少）。此外，急性运动降低了比目鱼肌中的 GSH/GSSG 比值，肌酸补充可预防这种降低（比目鱼肌：pre 8%，0 h 29%，2 h 30%和 6 h 44%，%预防）。结果表明，肌酸补充抑制了急性运动引起的血浆和肌肉中氧化应激标志物的增加。
