Gur'ianov I D, Karamova N S, Iusupova D V, Gnezdilov O I, Koshkarova L A
Bioorg Khim. 2013 Jan-Feb;39(1):121-8. doi: 10.7868/s0132342312060048.
The prodigiosin preparation was isolated and purified from Serratia marcescens ATCC 9986, using chromatographic methods. The analysis of the preparation by TLC, NMR-spectrometry and mass-spectrometry allowed to confirm the red pigment fraction as the prodigiosin and detect its purity. Originally, the specific features of the toxic and genotoxic effects of prodigiosin and the possibility of induction of mutations by pigment in the cells of Salmonella typhimurium TA 100 (Ames test) and chromosome damage of mammalian erythroblasts have been determined.
采用色谱法从粘质沙雷氏菌ATCC 9986中分离并纯化灵菌红素制剂。通过薄层色谱法（TLC）、核磁共振光谱法（NMR）和质谱分析法对该制剂进行分析，证实红色色素部分为灵菌红素，并检测其纯度。最初，已确定灵菌红素的毒性和遗传毒性作用的具体特征，以及该色素在鼠伤寒沙门氏菌TA 100细胞中诱导突变的可能性（艾姆斯试验）和对哺乳动物成红细胞染色体的损伤情况。
