Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan.
Atmosphere and Ocean Research Institute, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8564, Japan.
Int J Syst Evol Microbiol. 2012 Aug;62(Pt 8):1784-1789. doi: 10.1099/ijs.0.031443-0. Epub 2011 Oct 10.
Two coccoid, non-motile bacteria were isolated from seawater in the north-western Pacific Ocean near Japan. The two strains, designated S1-36(T) and S1-72(T), were Gram-negative, obligately aerobic, heterotrophic and catalase-negative. They were able to reduce nitrate to nitrogen. Both strains required NaCl for growth, with optimum growth in 2% NaCl, and grew at 15-30 °C, with optimum growth at 20-25 °C. Genomic DNA G+C contents of strains S1-36(T) and S1-72(T) were 59.6 and 59.4 mol%, respectively. The predominant isoprenoid quinone was Q-8 and major cellular fatty acids were C(16:1)ω7c, C(18:1)ω7c and C(17:1)ω8c. Analyses of 16S rRNA gene sequences revealed that strains S1-36(T) and S1-72(T) were related to each other (96.1% sequence similarity) and both strains showed 92.3-94.7% sequence similarity with members of the genus Haliea. On the basis of phenotypic and phylogenetic features, strains S1-36(T) and S1-72(T) should be classified as representatives of two novel species in a new genus, Halioglobus gen. nov., within the class Gammaproteobacteria. The names proposed are Halioglobus japonicus sp. nov., the type species of the genus, with S1-36(T) ( = NBRC 107739(T) = KCTC 23429(T)) as type strain, and Halioglobus pacificus sp. nov., with S1-72(T) ( = NBRC 107742(T) = KCTC 23430(T)) as type strain.
两株圆球形、非运动性细菌从日本西北部太平洋海域的海水中分离得到。这两株菌被命名为 S1-36(T)和 S1-72(T)，革兰氏阴性，严格需氧，异养，过氧化氢酶阴性。它们能够将硝酸盐还原为氮气。两株菌均需要 NaCl 才能生长，最适生长在 2% NaCl 中，生长温度为 15-30°C，最适生长温度为 20-25°C。菌株 S1-36(T)和 S1-72(T)的基因组 DNA G+C 含量分别为 59.6 和 59.4 mol%。主要的异戊二烯醌为 Q-8，主要的细胞脂肪酸为 C(16:1)ω7c、C(18:1)ω7c 和 C(17:1)ω8c。16S rRNA 基因序列分析表明，菌株 S1-36(T)和 S1-72(T)彼此相关（序列相似度为 96.1%），并且两株菌与 Haliea 属的成员的序列相似度为 92.3-94.7%。基于表型和系统发育特征，菌株 S1-36(T)和 S1-72(T)应被归类为一个新属的两个新种，即 Haliea 属的代表，属于γ变形菌纲。建议的名称是日本海氏菌（Halioglobus japonicus），模式种，以 S1-36(T)（=NBRC 107739(T)=KCTC 23429(T)）作为模式株，以及太平洋海氏菌（Halioglobus pacificus），以 S1-72(T)（=NBRC 107742(T)=KCTC 23430(T)）作为模式株。
