Yokohama Plant Protection Station, 1-16-10 Shinyamashita, Naka-ku, Yokohama, Kanagawa, Japan.
Yamagata General Agricultural Research Center, 6060-27 Minorigaoka, Yamagata, Japan.
Int J Syst Evol Microbiol. 2012 Aug;62(Pt 8):1799-1803. doi: 10.1099/ijs.0.032011-0. Epub 2011 Oct 10.
Bacteria were isolated from black lesions on shoots of European pear trees (Pyrus communis L.) in an orchard in Japan. Previous characterization of this novel pathogen by phenotypic and genotypic methods suggested that it should belong to the genus Erwinia but might not correspond to either Erwinia amylovora or Erwinia pyrifoliae. Here, phylogenetic analyses of the 16S rRNA gene, gyrB, and rpoD gene sequences indicated that it could not be assigned to any recognized species of the genus Erwinia. DNA-DNA hybridization confirmed that the bacterial strains represented a novel species. The DNA G+C contents, the fatty acid profile and phenotypic characteristics resembled those previously reported for members of the genus Erwinia. On the basis of these and previous results, the pathogen represents a novel species of the genus Erwinia, for which the name Erwinia uzenensis sp. nov. (type strain: YPPS 951(T) = LMG 25843(T) = NCPPB 4475(T)) is proposed.
从日本果园中欧洲梨（Pyrus communis L.）的嫩枝上的黑色病斑中分离出细菌。先前通过表型和基因型方法对这种新型病原体的特征描述表明，它应属于欧文氏菌属，但与梨火疫欧文氏菌或李欧文氏菌均不对应。在此，16S rRNA 基因、gyrB 和 rpoD 基因序列的系统发育分析表明，它不能被分配到欧文氏菌属的任何公认种。DNA-DNA 杂交证实，细菌株代表了一个新种。DNA G+C 含量、脂肪酸图谱和表型特征与先前报道的欧文氏菌属成员相似。基于这些和先前的结果，该病原体代表了欧文氏菌属的一个新种，将其命名为欧文氏菌属 Uzenensis 种。nov.（模式株：YPPS 951(T)=LMG 25843(T)=NCPPB 4475(T)）。
