Transfer of and to a novel genus gen. nov. as comb. nov. and comb. nov. and description of sp. nov., isolated from bleeding cankers on broadleaf hosts.

Following a screening campaign of bleeding cankers of broadleaf hosts in Great Britain, numerous bacterial strains were isolated, identified by 16S rRNA and protein-coding gene sequencing and ultimately classified. During the course of the study, several Gram-negative, facultatively anaerobic strains were isolated from bleeding x (London plane) and x (common lime) cankers that could not be assigned to an existing species. Partial 16S rRNA gene sequencing placed these strains in the genus , as a close phylogenetic relative of . In an effort to determine the taxonomic position of the strains, a polyphasic approach was followed including genotypic, genomic, phenotypic, and chemotaxonomic assays. Multilocus sequence analysis based on four protein-coding genes (, , , and ) confirmed the phylogenetic position of the strains as a novel taxon of subgroup 3 of the genus , along with and , and furthermore, provided support for their reclassification in a novel genus. Whole genome comparisons allowed the delimitation of the novel species and also supported the proposed transfer of subgroup 3 species to a novel genus in the . Phenotypically the novel species could be differentiated from and , and the novel genus could be differentiated from the closely related genera and . Therefore, we propose (1) the reclassification of and in a novel genus, gen. nov., as comb. nov. and comb. nov., with comb. nov. as the type species (type strain A37 = CFBP 6631 = ATCC 700880 = CECT 5263), and (2) classification of the novel strains as sp. nov. (type strain BAC 15a-03b = LMG 32576 = NCPPB 4696).