Center for Inflammatory Bowel Disease - Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA.
Inflamm Bowel Dis. 2012 May;18(5):935-42. doi: 10.1002/ibd.21874. Epub 2011 Oct 10.
Oral pathology is a commonly reported extraintestinal manifestation of Crohn's disease (CD). The host-microbe interaction has been implicated in the pathogenesis of inflammatory bowel disease (IBD) in genetically susceptible hosts, yet limited information exists about oral microbes in IBD. We hypothesize that the microbiology of the oral cavity may differ in patients with IBD. Our laboratory has developed a 16S rRNA-based technique known as the Human Oral Microbe Identification Microarray (HOMIM) to study the oral microbiome of children and young adults with IBD.
Tongue and buccal mucosal brushings from healthy controls, CD, and ulcerative colitis (UC) patients were analyzed using HOMIM. Shannon Diversity Index (SDI) and Principal Component Analysis (PCA) were employed to compare population and phylum-level changes among our study groups.
In all, 114 unique subjects from the Children's Hospital Boston were enrolled. Tongue samples from patients with CD showed a significant decrease in overall microbial diversity as compared with the same location in healthy controls (P = 0.015) with significant changes seen in Fusobacteria (P < 0.0002) and Firmicutes (P = 0.022). Tongue samples from patients with UC did not show a significant change in overall microbial diversity as compared with healthy controls (P = 0.418).
As detected by HOMIM, we found a significant decrease in overall diversity in the oral microbiome of pediatric CD. Considering the proposed microbe-host interaction in IBD, the ease of visualization and direct oral mucosal sampling of the oral cavity, further study of the oral microbiome in IBD is of potential diagnostic and prognostic value.
口腔病理学是克罗恩病（CD）常见的肠道外表现。宿主-微生物相互作用被认为与遗传易感宿主的炎症性肠病（IBD）发病机制有关，但关于 IBD 患者口腔微生物的信息有限。我们假设 IBD 患者口腔微生物学可能存在差异。我们的实验室开发了一种基于 16S rRNA 的技术，称为人类口腔微生物鉴定微阵列（HOMIM），用于研究 IBD 儿童和年轻成人的口腔微生物组。
使用 HOMIM 分析健康对照者、CD 和溃疡性结肠炎（UC）患者的舌和颊黏膜刷检样本。采用 Shannon 多样性指数（SDI）和主成分分析（PCA）比较我们研究组之间的种群和门水平变化。
共纳入 114 名来自波士顿儿童医院的独特受试者。与健康对照组相比，CD 患者的舌样本的整体微生物多样性明显降低（P = 0.015），梭杆菌（P < 0.0002）和厚壁菌门（Firmicutes）（P = 0.022）显著变化。与健康对照组相比，UC 患者的舌样本的整体微生物多样性没有明显变化（P = 0.418）。
通过 HOMIM 检测，我们发现儿科 CD 患者口腔微生物组的整体多样性明显降低。考虑到 IBD 中提出的微生物-宿主相互作用，以及 IBD 口腔微生物组具有可视化和直接口腔黏膜采样的优势，进一步研究 IBD 的口腔微生物组具有潜在的诊断和预后价值。
