具有淋巴结转移潜能的胃癌潜在预测 microRNAs。
Potentially predictive microRNAs of gastric cancer with metastasis to lymph node.
机构信息
Department of General Surgery, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China.
出版信息
World J Gastroenterol. 2011 Aug 21;17(31):3645-51. doi: 10.3748/wjg.v17.i31.3645.
AIM
To detect the expression of 60 microRNAs (miRNAs) in gastric cancer tissues and find new predictive biomarkers of gastric cancer with metastasis.
METHODS
The expressions of 60 candidate miRNAs in 30 gastric cancer tissues and paired normal tissues were detected by stem-loop real-time reverse transcription-polymerase chain reaction. After primary screening of miRNAs expression, 5 selected miRNAs were further testified in another 22 paired gastric tissues. Based on the expression level of miRNAs and the status of metastasis to lymph node (LN), receiver-operating-characteristic (ROC) curve were used to evaluate their ability in predicting the status of metastasis to LN.
RESULTS
Thirty-eight miRNAs expressions in gastric cancer tissues were significantly different from those in paired normal tissues (P < 0.01). Among them, 31 miRNAs were found to be up-expressed in cancer tissues and 1 miRNAs were down-expressed ≥ 1.5 fold vs paired normal gastric tissue. Five microRNAs (miR-125a-3p, miR-133b, miR-143, miR-195 and miR-212) were differently expressed between different metastatic groups in 30 gastric cancer biopsies (P < 0.05). Partial correlation analysis showed that hsa-mir-212 and hsa-mir-195 were correlated with the status of metastasis to LN in spite of age, gender, tumor location, tumor size, depth of invasion and cell differentiation. ROC analysis indicated that miR-212 and miR-195 have better sensitivities (84.6% and 69.2%, respectively) and specificities (both 100%) in distinguishing biopsies with metastasis to LN from biopsies without metastasis to LN.
CONCLUSION
miR-212 and miR-195 could be independent biomarkers in predicting the gastric cancer with metastasis to LN.
目的
检测 60 种微小 RNA（miRNA）在胃癌组织中的表达，寻找具有转移性胃癌的新预测生物标志物。
方法
采用茎环实时逆转录聚合酶链反应检测 30 例胃癌组织和配对正常组织中 60 种候选 miRNA 的表达。miRNA 表达初步筛选后，在另 22 对配对胃癌组织中进一步验证 5 种选定的 miRNA。根据 miRNA 的表达水平和淋巴结转移（LN）的状态，绘制受试者工作特征（ROC）曲线，评估其预测 LN 转移状态的能力。
结果
38 种 miRNA 在胃癌组织中的表达与配对正常组织中的表达有显著差异（P<0.01）。其中，31 种 miRNA 在癌组织中表达上调，1 种 miRNA 表达下调≥1.5 倍。在 30 例胃癌活检中，5 种 miRNA（miR-125a-3p、miR-133b、miR-143、miR-195 和 miR-212）在不同转移性组之间表达不同（P<0.05）。偏相关分析显示，hsa-mir-212 和 hsa-mir-195 与 LN 转移状态相关，不受年龄、性别、肿瘤位置、肿瘤大小、浸润深度和细胞分化的影响。ROC 分析表明，miR-212 和 miR-195 对区分有 LN 转移的活检和无 LN 转移的活检具有更好的敏感性（分别为 84.6%和 69.2%）和特异性（均为 100%）。
结论
miR-212 和 miR-195 可能是预测具有 LN 转移的胃癌的独立生物标志物。
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