Adeleke O E, Coker M E, Oke O B
Department of Pharmaceutical Microbiology, University of Ibadan, Ibadan, Nigeria.
Ann Burns Fire Disasters. 2010 Jun 30;23(2):102-5.
Studies on Staphylococcusaureus and Staphylococcusintermedius from dog and cat, and also on Staphylococcusaureus from wound and pyoderma infections, have shown a correlation between the site of microbial infection and antimicrobial susceptibility. Both the methanolic extract concentrate of Garcinia kola (Heckel) seeds and natural honey have been associated with activity on bacterial isolates from respiratory tract infections. In this study, selected bacteria belonging to genera from burn wound infection sites were treated with natural honey and methanolic extract concentrate of Garcinia kola in antimicrobial susceptibility tests separately and in combined form, and also with gentamicin and methanol as controls. The two natural products were found to be active on the bacterial isolates, excluding Klebsiellapneumoniae strains, all of which showed resistance to honey. Combination forms of the two natural products were active only on the strains of Pseudomonasaeruginosa. At 4 and 8 µg/ml, gentamicin was ineffective on the three strains of Klebsiellapneumoniae while 8 µg/ml was moderately active on only two strains of Pseudomonasaeruginosa. One strain of Pseudomonasaeruginosa, UCH002, was resistant to gentamicin beyond 1,000 µ/ml. Gentamicin at 4 µ/ml was inhibitory to one strain of Escherichiacoli and two strains of Staphylococcusaureus. Though the antimicrobial activity of the two natural products tested had been previously reported against microbial agents of respiratory tract infection, it was also recorded in this study. The lack of activity of each of the three honey types used in this study against the Klebsiellapneumoniae strains tested underscores the need to exclude this organism from burn wound infections before embarking on treatment with honey. The sensitivity of one high-level gentamicin-resistant strain of Pseudomonasaeruginosa to honey and Garcinia kola seed extract was noteworthy considering the therapeutic failures of gentamicin and other antibiotics against Pseudomonasaeruginosa.
对犬猫源金黄色葡萄球菌和中间葡萄球菌，以及伤口和脓皮病感染源金黄色葡萄球菌的研究表明，微生物感染部位与抗菌药敏性之间存在关联。可乐果（Heckel）种子的甲醇提取物浓缩物和天然蜂蜜均与呼吸道感染细菌分离株的活性有关。在本研究中，将来自烧伤伤口感染部位的选定属细菌分别及联合用天然蜂蜜和可乐果甲醇提取物浓缩物进行抗菌药敏试验，并以庆大霉素和甲醇作为对照。发现这两种天然产物对细菌分离株有活性，但肺炎克雷伯菌菌株除外，所有肺炎克雷伯菌菌株均对蜂蜜耐药。两种天然产物的联合形式仅对铜绿假单胞菌菌株有活性。在4和8μg/ml时，庆大霉素对三株肺炎克雷伯菌无效，而8μg/ml仅对两株铜绿假单胞菌有中等活性。一株铜绿假单胞菌UCH002对庆大霉素的耐药浓度超过1000μg/ml。4μg/ml的庆大霉素对一株大肠杆菌和两株金黄色葡萄球菌有抑制作用。尽管之前已报道过所测试的这两种天然产物对呼吸道感染微生物制剂具有抗菌活性，但本研究中也有记录。本研究中使用的三种蜂蜜对所测试的肺炎克雷伯菌菌株均无活性，这突出表明在开始用蜂蜜治疗之前，需要将该菌从烧伤伤口感染中排除。考虑到庆大霉素和其他抗生素对铜绿假单胞菌治疗失败，一株高水平庆大霉素耐药的铜绿假单胞菌对蜂蜜和可乐果种子提取物敏感值得关注。
