Department of Botany, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Mol Ecol. 2011 Sep;20(17):3499-502. doi: 10.1111/j.1365-294x.2011.05197.x.
The first North American RAD Sequencing and Genomics Symposium, sponsored by Floragenex (http://www.floragenex.com/radmeeting/), took place in Portland, Oregon (USA) on 19 April 2011. This symposium was convened to promote and discuss the use of restriction-site-associated DNA (RAD) sequencing technologies. RAD sequencing is one of several strategies recently developed to increase the power of data generated via short-read sequencing technologies by reducing their complexity (Baird et al. 2008; Huang et al. 2009; Andolfatto et al. 2011; Elshire et al. 2011). RAD sequencing, as a form of genotyping by sequencing, has been effectively applied in genetic mapping and quantitative trait loci (QTL) analyses in a range of organisms including nonmodel, genetically highly heterogeneous organisms (Table 1; Baird et al. 2008; Baxter et al. 2011; Chutimanitsakun et al. 2011; Pfender et al. 2011). RAD sequencing has recently found applications in phylogeography (Emerson et al. 2010) and population genomics (Hohenlohe et al. 2010). Considering the diversity of talks presented during this meeting, more developments are to be expected in the very near future.
北美首个 RAD 测序与基因组学研讨会于 2011 年 4 月 19 日在美国俄勒冈州波特兰市举行，由 Floragenex（http://www.floragenex.com/radmeeting/）主办。本次研讨会旨在促进和讨论限制位点相关 DNA（RAD）测序技术的应用。RAD 测序是最近开发的几种策略之一，通过降低其复杂性来提高通过短读测序技术生成数据的能力（Baird 等人，2008 年；Huang 等人，2009 年；Andolfatto 等人，2011 年；Elshire 等人，2011 年）。RAD 测序作为一种测序基因型的方法，已在包括非模型、遗传高度异质的生物体在内的一系列生物体的遗传图谱和数量性状位点（QTL）分析中得到有效应用（表 1；Baird 等人，2008 年；Baxter 等人，2011 年；Chutimanitsakun 等人，2011 年；Pfender 等人，2011 年）。RAD 测序最近已应用于系统地理学（Emerson 等人，2010 年）和种群基因组学（Hohenlohe 等人，2010 年）。考虑到本次会议上提出的演讲多样性，预计在不久的将来还会有更多的发展。
