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重组毕赤酵母利用木糖高效生产 L-乳酸。

Efficient production of L-lactic acid from xylose by a recombinant Candida utilis strain.

机构信息

Central Laboratories for Frontier Technology, KIRIN Holdings Co., Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama-shi, Kanagawa 236-0004, Japan.

出版信息

J Biosci Bioeng. 2012 Jan;113(1):73-5. doi: 10.1016/j.jbiosc.2011.09.002. Epub 2011 Oct 12.

DOI:10.1016/j.jbiosc.2011.09.002
PMID:21996028
Abstract

Efficient L-lactic acid production from xylose was achieved using a pyruvate decarboxylase-deficient Candida utilis strain expressing an L-lactate dehydrogenase, an NADH-preferring mutated xylose reductase (XR), a xylitol dehydrogenase and a xylulokinase. The recombinant strain showed 53% increased L-lactic acid production compared with the reference strain expressing native XR (NADPH-preferring).

摘要

利用一株缺乏丙酮酸脱羧酶的产朊假丝酵母（Candida utilis）工程菌，通过表达 L-乳酸脱氢酶、NADH 偏好型突变木糖还原酶（XR）、木糖醇脱氢酶和木酮糖激酶，实现了从木糖到 L-乳酸的高效生产。与表达天然 XR（NADPH 偏好型）的参比菌株相比，重组菌株的 L-乳酸产量提高了 53%。

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