Division of Pediatric Dentistry, Department of Dental Medicine, Karolinska Institutet, Stockholm, Sweden.
Obesity (Silver Spring). 2012 Jan;20(1):157-64. doi: 10.1038/oby.2011.305. Epub 2011 Oct 13.
To test the hypothesis whether microbiota in oral biofilm is linked with obesity in adolescents we designed this cross-sectional study. Obese adolescents (n = 29) with a mean age of 14.7 years and normal weight subjects (n = 58) matched by age and gender were examined with respect to visible plaque index (VPI%) and gingival inflammation (bleeding on probing (BOP%)). Stimulated saliva was collected. They answered a questionnaire concerning medical history, medication, oral hygiene habits, smoking habits, and sociodemographic background. Microbiological samples taken from the gingival crevice was analyzed by checkerboard DNA-DNA hybridization technique. The sum of bacterial cells in subgingival biofilm was significantly associated with obesity (P < 0.001). The link between sum of bacterial cells and obesity was not confounded by any of the studied variables (chronic disease, medication, VPI%, BOP%, flow rate of whole saliva, or meal frequency). Totally 23 bacterial species were present in approximately threefold higher amounts, on average, in obese subjects compared with normal weight controls. Of the Proteobacteria phylum, Campylobacter rectus and Neisseria mucosa were present in sixfold higher amounts among obese subjects. The association between obesity and sum of bacterial cells in oral subgingival biofilm indicates a possible link between oral microbiota and obesity in adolescents.
为了检验口腔生物膜中的微生物群是否与青少年肥胖有关的假设，我们设计了这项横断面研究。研究纳入了 29 名平均年龄为 14.7 岁的肥胖青少年和 58 名年龄和性别相匹配的正常体重受试者，分别评估其可见牙菌斑指数（VPI%）和牙龈炎症（探诊出血（BOP%））。收集了刺激唾液。他们回答了一份关于病史、用药、口腔卫生习惯、吸烟习惯和社会人口背景的问卷。通过斑点杂交 DNA-DNA 杂交技术分析从牙龈沟中采集的微生物样本。龈下生物膜中细菌细胞的总和与肥胖显著相关（P < 0.001）。细菌细胞总和与肥胖之间的联系不受任何研究变量（慢性病、用药、VPI%、BOP%、全唾液流速或进餐频率）的干扰。在肥胖受试者中，平均有 23 种细菌的含量约高出三倍，与正常体重对照组相比。在变形菌门中，直肠弯曲杆菌和粘膜奈瑟菌在肥胖受试者中的含量高出六倍。肥胖与口腔龈下生物膜中细菌细胞总和之间的关联表明口腔微生物群与青少年肥胖之间可能存在联系。
