Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
J Sep Sci. 2011 Nov;34(21):3107-13. doi: 10.1002/jssc.201100455. Epub 2011 Oct 14.
The aerial parts of Hypericum carinatum (Guttiferae) were extracted with supercritical carbon dioxide under constant temperature (40, 50 or 60°C) and gradual pressure increase (90, 120, 150 and 200 bar) aiming at the recovery of enriched fractions containing uliginosin B, cariphenone A and cariphenone B, compounds of pharmaceutical interest. The yields of these substances were determined by high-performance liquid chromatography and compared with those obtained with n-hexane maceration. The supercritical-fluid extraction showed higher selectivity than the conventional solvent extraction method. After defining 40°C and 90 bar as the best conditions to obtain the target compounds, a mathematical model was used for the extraction process and a good correlation was achieved with the experimental data.
超基性岩（Guttiferae）的空中部分用超临界二氧化碳在恒温（40、50 或 60°C）和逐渐加压（90、120、150 和 200 巴）下进行提取，旨在回收含有 uliginosin B、cariphenone A 和 cariphenone B 的富分，这些化合物具有药用价值。这些物质的产率通过高效液相色谱法测定，并与正己烷浸取法的产率进行了比较。超临界流体萃取显示出比传统溶剂萃取方法更高的选择性。在确定 40°C 和 90 巴作为获得目标化合物的最佳条件后，对萃取过程进行了数学建模，并与实验数据取得了良好的相关性。
