Department of Endodontology, School of Dentistry, Oregon Health and Science University, Portland, Oregon 97239, USA.
J Endod. 2011 Nov;37(11):1542-6. doi: 10.1016/j.joen.2011.06.022. Epub 2011 Jul 30.
Endodontic repair materials such as mineral trioxide aggregate (MTA) are used for various endodontic procedures. An alternative material to MTA with purportedly improved handling properties is EndoSequence Root Repair Material, which is available as premixed putty (ESP) or syringeable paste (ESS) and is described as possessing antibacterial activity during its setting reaction due to its highly alkaline pH. The aim of this in vitro study was to determine whether ESP and ESS possess antibacterial properties against a collection of Enterococcus faecalis strains recovered from root canal infections. The hypotheses tested were that (1) ESP and ESS possess antibacterial activity during their setting reaction, (2) there is no difference between ESP, ESS, and MTA in antibacterial activity, and (3) E. faecalis strains isolated from root canals differ in susceptibility to the materials.
The direct contact test was used. ESP, ESS, and white MTA were preincubated at 37°C in >95% humidity for 30 minutes and 24 hours before 1-hour direct contact exposure to E. faecalis strains (n = 10). Absence of antibacterial carryover effect from the materials to the bacterial cultures was confirmed. Log(10) viable counts were compared by using analysis of variance with significance level at P ≤ .05.
Combining data for all strains, the mean (± standard deviation) log(10) viable counts for ESP (4.55 ± 0.85), ESS (4.5 ± 0.95), and MTA (4.12 ± 1.26) were significantly lower than for untreated controls (7.40 ± 0.33) (P < .0001). The reduction in viable counts ranged from 1.86 ± 0.24 to 4.78 ± 0.42, with no statistically significant differences between the materials or preincubation periods. One strain was significantly more susceptible than 4 other strains.
ESP, ESS, and MTA had similar antibacterial efficacy against clinical strains of E. faecalis. Clinical strains varied in their susceptibility to the root repair materials.
矿化三氧化物凝聚体（MTA）等牙髓修复材料用于各种牙髓治疗程序。一种据称具有改良操作性的 MTA 替代材料是 EndoSequence 根管修复材料，它有预混糊剂（ESP）和注射器型糊剂（ESS）两种形式，其说明书指出，由于其具有高碱性 pH 值，在凝固反应期间具有抗菌活性。本体外研究的目的是确定 ESP 和 ESS 是否对从根管感染中回收的一系列粪肠球菌菌株具有抗菌作用。检验的假设是：(1)ESP 和 ESS 在凝固反应期间具有抗菌活性；(2)ESP、ESS 和 MTA 在抗菌活性方面没有差异；(3)从根管中分离的粪肠球菌菌株对这些材料的敏感性不同。
使用直接接触试验。ESP、ESS 和白色 MTA 在 37°C 和 >95%湿度下预孵育 30 分钟和 24 小时，然后与粪肠球菌菌株（n=10）直接接触 1 小时。确认材料不会对细菌培养物产生抗菌性的延续作用。使用方差分析比较对数（10）活菌计数，显著性水平为 P≤0.05。
综合所有菌株的数据，ESP（4.55±0.85）、ESS（4.5±0.95）和 MTA（4.12±1.26）的平均（±标准差）对数（10）活菌计数明显低于未处理对照组（7.40±0.33）（P<0.0001）。活菌计数减少范围为 1.86±0.24 至 4.78±0.42，材料之间或预孵育时间之间无统计学差异。一种菌株明显比其他 4 种菌株更敏感。
ESP、ESS 和 MTA 对粪肠球菌临床菌株具有相似的抗菌效果。临床菌株对根管修复材料的敏感性不同。
