Hazen K C, LeMelle W G
Division of Biological Sciences, University of Montana, Missoula 59812-1201.
Appl Environ Microbiol. 1990 Jun;56(6):1974-6. doi: 10.1128/aem.56.6.1974-1976.1990.
A simple method that distinguishes among hydrophobic avidity levels of highly hydrophobic isolates of the pathogenic fungus Candida albicans is described. This method involves mixing polystyrene microspheres at different concentrations with a constant concentration of yeast cells and plotting the data in accordance with the Langmuir isotherm equation. A 10-fold difference between the C. albicans isolates with the lowest and highest avidity (KH) values was found. This method may also demonstrate that surface hydrophobic sites with different avidities are present within a yeast cell population.
描述了一种区分致病性真菌白色念珠菌高度疏水分离株疏水亲和力水平的简单方法。该方法包括将不同浓度的聚苯乙烯微球与恒定浓度的酵母细胞混合，并根据朗缪尔等温线方程绘制数据。发现白色念珠菌分离株中亲和力最低和最高（KH）值之间存在10倍的差异。该方法还可能表明酵母细胞群体中存在具有不同亲和力的表面疏水位点。
