共聚焦显微镜中的分辨率和光学切片。
Resolution and optical sectioning in the confocal microscope.
机构信息
Department of Engineering Science, University of Oxford, Oxford, U.K.
出版信息
J Microsc. 2011 Nov;244(2):113-21. doi: 10.1111/j.1365-2818.2011.03549.x.
Abstract
In this short review, we present a self-contained discussion of the image formation properties of the fluorescent confocal microscope. The optical sectioning or depth discrimination property is discussed in detail and new analytic formulae are presented, which relate the optical sectioning strength to the wavelength, numerical aperture and pinhole aperture size in a particularly simple fashion.
摘要
在这篇简短的综述中，我们对荧光共焦显微镜的成象特性进行了完整的讨论。详细讨论了光学切片或深度分辨特性，并提出了新的分析公式，这些公式以特别简单的方式将光学切片强度与波长、数值孔径和针孔孔径大小联系起来。
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