Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
J Food Prot. 2011 Oct;74(10):1729-34. doi: 10.4315/0362-028X.JFP-10-487.
Campylobacter jejuni is the most common bacterial cause of diarrheal disease in humans worldwide, with poultry products being a major source. Therefore, strategies to decrease Campylobacter colonization during primary production might aid in reducing the number of human campylobacteriosis cases. Several plant-derived compounds have been reported to possess anti-Campylobacter properties in vitro, so they could be promising candidates to reduce Campylobacter colonization in broiler chickens. To test this hypothesis, selected plant-derived antimicrobials (caffeic, gallic, protocatechuic, and vanillic acids, epigallocatechin gallate, trans-cinnamaldehyde, and thymol) were screened for anti-Campylobacter activity by determining MICs and setting up time-kill curves for C. jejuni strain KC 40. These experiments revealed marked antibacterial activity, especially for the cinnamon oil ingredient trans-cinnamaldehyde (CIN). This compound was tested in a broiler chick seeder model; it was added to the feed in coated form at an effective concentration of 0.3 % from day-of-hatch for the entire 22-day duration of the experiment. At 14 days of age, one-third of the birds were inoculated with C. jejuni strain KC 40 and served as seeders. CIN was not able to reduce cecal Campylobacter colonization in this model, which was confirmed in a cecal loop experiment. Despite CIN concentrations much higher than the MIC, C. jejuni numbers were not reduced compared with those in nontreated ceca at 2 and 24 h after injection. In conclusion, this study shows a marked discrepancy between in vitro and in vivo activity of CIN against C. jejuni strain KC 40.
空肠弯曲菌是全球人类腹泻病最常见的细菌性病因，而家禽产品是主要来源。因此，在初级生产过程中减少空肠弯曲菌定植的策略可能有助于减少人类弯曲菌病的病例数。已报道几种植物源性化合物在体外具有抗空肠弯曲菌的特性，因此它们可能是减少肉鸡空肠弯曲菌定植的有前途的候选物。为了验证这一假设，选择了几种植物源性抗菌剂（咖啡酸、没食子酸、原儿茶酸和香草酸、表没食子儿茶素没食子酸酯、反式肉桂醛和百里香酚），通过测定 MIC 并为 KC 40 空肠弯曲菌菌株建立时间杀伤曲线来筛选抗空肠弯曲菌活性。这些实验显示出明显的抗菌活性，特别是肉桂油成分反式肉桂醛（CIN）。该化合物在肉鸡播种模型中进行了测试；它以有效浓度 0.3%包被形式添加到饲料中，从孵出当天开始，整个 22 天的实验持续时间内都添加。在 14 日龄时，三分之一的鸡用 KC 40 空肠弯曲菌菌株接种，作为播种者。在这个模型中，CIN 不能减少盲肠空肠弯曲菌的定植，这在盲肠环实验中得到了证实。尽管 CIN 浓度远高于 MIC，但与未处理的盲肠相比，在注射后 2 和 24 小时，C. jejuni 数量并未减少。总之，这项研究表明 CIN 对 KC 40 空肠弯曲菌的体外和体内活性之间存在明显差异。
