卵泡促炎细胞因子和趋化因子作为体外受精成功的标志物
Follicular proinflammatory cytokines and chemokines as markers of IVF success.
作者信息
Sarapik Aili, Velthut Agne, Haller-Kikkatalo Kadri, Faure Gilbert C, Béné Marie-Christine, de Carvalho Bittencourt Marcelo, Massin Frédéric, Uibo Raivo, Salumets Andres
机构信息
Department of Immunology, Institute of General and Molecular Pathology, University of Tartu, Ravila 19, Tartu 50411, Estonia.
出版信息
Clin Dev Immunol. 2012;2012:606459. doi: 10.1155/2012/606459. Epub 2011 Oct 5.
Cytokines are key modulators of the immune system and also contribute to regulation of the ovarian cycle. In this study, Bender MedSystems FlowCytomix technology was used to analyze follicular cytokines (proinflammatory: IL-1β, IL-6, IL-18, IFN-γ, IFN-α, TNF-α, IL-12, and IL-23;, and anti-inflammatory: G-CSF), chemokines (MIP-1α, MIP-1β, MCP-1, RANTES, and IL-8), and other biomarkers (sAPO-1/Fas, CD44(v6)) in 153 women undergoing in vitro fertilization (IVF). Cytokine origin was studied by mRNA analysis of granulosa cells. Higher follicular MIP-1α and CD44(v6) were found to correlate with polycystic ovary syndrome, IL-23, INF-γ, and TNF-α with endometriosis, higher CD44(v6) but lower IL-β and INF-α correlated with tubal factor infertility, and lower levels of IL-18 and CD44(v6) characterized unexplained infertility. IL-12 positively correlated with oocyte fertilization and embryo development, while increased IL-18, IL-8, and MIP-1β were associated with successful IVF-induced pregnancy.
细胞因子是免疫系统的关键调节因子，也有助于调节卵巢周期。在本研究中，使用Bender MedSystems流式细胞混合技术分析了153名接受体外受精（IVF）的女性卵泡中的细胞因子（促炎因子：IL-1β、IL-6、IL-18、IFN-γ、IFN-α、TNF-α、IL-12和IL-23；抗炎因子：G-CSF）、趋化因子（MIP-1α、MIP-1β、MCP-1、RANTES和IL-8）以及其他生物标志物（sAPO-1/Fas、CD44(v6)）。通过对颗粒细胞进行mRNA分析来研究细胞因子的来源。研究发现，卵泡中较高的MIP-1α和CD44(v6)与多囊卵巢综合征相关，IL-23、INF-γ和TNF-α与子宫内膜异位症相关，较高的CD44(v6)但较低的IL-β和INF-α与输卵管因素不孕相关，而较低水平的IL-18和CD44(v6)是不明原因不孕的特征。IL-12与卵母细胞受精和胚胎发育呈正相关，而IL-18、IL-8和MIP-1β水平升高与IVF诱导的成功妊娠相关。
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