Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Iksan, Chonbuk, 570-749, Korea.
Eur J Clin Microbiol Infect Dis. 2012 Jul;31(7):1347-52. doi: 10.1007/s10096-011-1448-y. Epub 2011 Oct 19.
Streptococcus mutans is the key causative agent of caries and infective endocarditis. The first step in biofilm development and the consequent initiation of further disease is bacterial adherence to host cell surfaces. Therefore, the aim of this study was to evaluate the inhibitory effect of curcumin on S. mutans adherence to extracellular matrices and tooth surfaces. The effect of curcumin on the ability of S. mutans to adhere to glass surfaces coated with collagen and fibronectin was tested in order to determine whether the decrease of the bacterial adhesion by curcumin is achieved by hindering the bacteria in adhering to collagen and/or fibronectin. Also, human teeth inoculated with S. mutans were treated with curcumin in vitro in order to assess the relevance of the anti-adhesive effect to oral conditions in vivo. The minimum inhibitory concentration (MIC) at which curcumin completely inhibited bacterial growth was 128 μg/mL. The addition of curcumin below the MIC diminished bacterial adherence onto both collagen- and fibronectin-coated glass surfaces and human tooth surfaces. It appears that the anti-adhesive effect of curcumin against S. mutans is mediated through collagen and fibronectin. These results support the widespread use of curcumin as a food-based antimicrobial agent.
变形链球菌是龋齿和感染性心内膜炎的主要致病菌。生物膜形成和随后疾病发生的第一步是细菌黏附于宿主细胞表面。因此，本研究旨在评估姜黄素对变形链球菌黏附于细胞外基质和牙齿表面的抑制作用。通过检测姜黄素对黏附于涂有胶原蛋白和纤维连接蛋白的玻璃表面的变形链球菌的黏附能力的影响，以确定姜黄素降低细菌黏附能力是通过阻止细菌黏附于胶原蛋白和/或纤维连接蛋白来实现的。此外，体外用人牙接种变形链球菌，并用姜黄素处理，以评估其抗黏附作用与体内口腔条件的相关性。完全抑制细菌生长的姜黄素最小抑菌浓度（MIC）为 128μg/mL。低于 MIC 的姜黄素的添加减少了细菌黏附于涂有胶原蛋白和纤维连接蛋白的玻璃表面和人牙表面。姜黄素对变形链球菌的抗黏附作用似乎是通过胶原蛋白和纤维连接蛋白介导的。这些结果支持将姜黄素广泛用作基于食品的抗菌剂。
