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小麦(Triticum aestivum L.)中小非编码 RNA 的探索。

Exploration of small non coding RNAs in wheat (Triticum aestivum L.).

机构信息

State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China.

出版信息

Plant Mol Biol. 2012 Sep;80(1):67-73. doi: 10.1007/s11103-011-9835-4. Epub 2011 Oct 19.

Abstract

Large numbers of noncoding RNA transcripts (ncRNAs) are being revealed in animals and plants, which can function at the transcriptional or posttranscriptional level to negatively regulate or control genes, repetitive sequences, viruses, and mobile elements. With the identification of microRNA and siRNAs in diverse organisms, increasing evidences indicate that these short npcRNAs play important roles in development, stress response and diseases by cleavage of target mRNA or interfere with translation of target genes. To explore the small RNA transcriptome in wheat (Triticum aestivum L.), a couple of small RNA libraries were constructed and sequenced by high throughput sequencing method. In this review, we focused on the discovery of wheat small RNAs including miRNA and some other non coding small RNAs, then have a view of miRNAs conservations and differences among wheat and other plant species. We also summarized the developmental and stress responsive expression of wheat miRNAs and these observations could serve as a foundation for future functional studies.

摘要

大量的非编码 RNA 转录本(ncRNAs)在动植物中被揭示出来,它们可以在转录或转录后水平上发挥作用,负调控或控制基因、重复序列、病毒和移动元件。随着在不同生物中鉴定出 microRNA 和 siRNAs,越来越多的证据表明这些短的 ncRNAs 通过切割靶 mRNA 或干扰靶基因的翻译,在发育、应激反应和疾病中发挥重要作用。为了探索小麦(Triticum aestivum L.)中的小 RNA 转录组,我们构建了几个小 RNA 文库,并通过高通量测序方法进行了测序。在这篇综述中,我们重点介绍了小麦小 RNA 的发现,包括 miRNA 和一些其他非编码小 RNA,然后观察了 miRNA 在小麦和其他植物物种中的保守性和差异。我们还总结了小麦 miRNA 在发育和应激反应中的表达情况,这些观察结果可以为未来的功能研究提供基础。

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