Shimomura Y, Takahashi M, Shimizu H, Sato N, Uehara Y, Negishi M, Inukai T, Kobayashi I, Kobayashi S
Department of Medicine, Gunma University of Medicine, Maebashi, Japan.
Physiol Behav. 1990 Apr;47(4):731-4. doi: 10.1016/0031-9384(90)90086-j.
The present studies were undertaken to investigate whether or not decreased ambulatory activity, including abnormal feeding behavior in diabetic rats, will be simultaneously normalized by insulin administration. To do this, we used the Gunma University-type automatic apparatus for continuous and direct measurement of ambulation and drinking. In this study, 3 U NPH insulin were administered at 1800, just before the dark phase, and 2 U were administered at 0600, just before the light phase. With these insulin doses, we found that 5 weeks were needed to normalize ambulatory activity, 4 weeks were necessary for food intake, 6 weeks for drinking and 2 weeks for body weight. Since ambulatory activity is reported to be related to changes in dopamine turnover, further studies are in progress to determine whether or not dopamine turnover is normalized when there is no difference in ambulatory activity due to insulin replacement.
本研究旨在探讨给予胰岛素后，糖尿病大鼠包括异常摄食行为在内的活动减少是否会同时恢复正常。为此，我们使用群马大学型自动装置连续直接测量活动和饮水情况。在本研究中，在黑暗期开始前的18:00给予3单位的中效胰岛素（NPH），在光照期开始前的06:00给予2单位。使用这些胰岛素剂量，我们发现活动恢复正常需要5周，食物摄入量恢复正常需要4周，饮水恢复正常需要6周，体重恢复正常需要2周。由于据报道活动与多巴胺代谢变化有关，目前正在进行进一步研究，以确定在胰岛素替代后活动无差异时，多巴胺代谢是否恢复正常。
