内镜成像：我们距离实时组织学还有多远？
Endoscopic imaging: How far are we from real-time histology?
作者信息
Shukla Richa, Abidi Wasif M, Richards-Kortum Rebecca, Anandasabapathy Sharmila
机构信息
Richa Shukla, Wasif M Abidi, Sharmila Anandasabapathy, Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, United States.
出版信息
World J Gastrointest Endosc. 2011 Oct 16;3(10):183-94. doi: 10.4253/wjge.v3.i10.183.
Abstract
Currently, in gastrointestinal endoscopy there is increasing interest in high resolution endoscopic technologies that can complement high-definition white light endoscopy by providing real-time subcellular imaging of the epithelial surface. These 'optical biopsy' technologies offer the potential to improve diagnostic accuracy and yield, while facilitating real-time decision-making. Although many endoscopic techniques have preliminarily shown high accuracy rates, these technologies are still evolving. This review will provide an overview of the most promising high-resolution imaging technologies, including high resolution microendoscopy, optical coherence tomography, endocytoscopy and confocal laser endoscopy. This review will also discuss the application and current limitations of these technologies for the early detection of neoplasia in Barrett's esophagus, ulcerative colitis and colorectal cancer.
摘要
目前，在胃肠内镜检查中，人们对高分辨率内镜技术的兴趣与日俱增，这些技术能够通过提供上皮表面的实时亚细胞成像来补充高清白光内镜检查。这些“光学活检”技术有潜力提高诊断准确性和诊断率，同时有助于实时决策。尽管许多内镜技术初步显示出高准确率，但这些技术仍在不断发展。本综述将概述最具前景的高分辨率成像技术，包括高分辨率显微内镜、光学相干断层扫描、细胞内镜和共聚焦激光内镜。本综述还将讨论这些技术在巴雷特食管、溃疡性结肠炎和结直肠癌肿瘤早期检测中的应用及当前局限性。
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本文引用的文献
1
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Technol Cancer Res Treat. 2011 Oct;10(5):431-41. doi: 10.7785/tcrt.2012.500220.
2
New endoscopic approaches in IBD.炎症性肠病的新型内镜治疗方法。
World J Gastroenterol. 2011 Jan 7;17(1):63-8. doi: 10.3748/wjg.v17.i1.63.
3
In vivo, dual-modality OCT/LIF imaging using a novel VEGF receptor-targeted NIR fluorescent probe in the AOM-treated mouse model.体内，使用新型 VEGF 受体靶向近红外荧光探针在 AOM 处理的小鼠模型中进行的双模态 OCT/LIF 成像。
Mol Imaging Biol. 2011 Dec;13(6):1173-82. doi: 10.1007/s11307-010-0450-6.
4
Molecular imaging of VEGF in gastrointestinal cancer in vivo using confocal laser endomicroscopy.应用共聚焦激光内镜术对胃肠道癌中 VEGF 的分子成像。
Gut. 2010 Aug;59(8):1046-55. doi: 10.1136/gut.2009.202986.
5
Endoscopic techniques for recognizing neoplasia in Barrett's esophagus: which should the clinician use?内镜技术在 Barrett 食管中识别肿瘤：临床医生应该使用哪种技术？
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6
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Gastroenterology. 2010 Aug;139(2):388-92, 392.e1-2. doi: 10.1053/j.gastro.2010.06.029. Epub 2010 Jun 15.
7
Role of confocal endomicroscopy in the diagnosis of celiac disease.共聚焦内镜在腹腔疾病诊断中的作用。
J Pediatr Gastroenterol Nutr. 2010 Sep;51(3):274-9. doi: 10.1097/MPG.0b013e3181d1b02c.
8
Epidemiology of gastrointestinal and liver tumors.胃肠道和肝脏肿瘤的流行病学。
Eur Rev Med Pharmacol Sci. 2010 Apr;14(4):249-58.
9
Evaluation of quantitative image analysis criteria for the high-resolution microendoscopic detection of neoplasia in Barrett's esophagus.评价高分辨率微内镜检测 Barrett 食管中肿瘤的定量图像分析标准。
J Biomed Opt. 2010 Mar-Apr;15(2):026027. doi: 10.1117/1.3406386.
10
The safety of intravenous fluorescein for confocal laser endomicroscopy in the gastrointestinal tract.经静脉注射荧光素用于胃肠道共聚焦激光内镜检查的安全性。
Aliment Pharmacol Ther. 2010 Mar;31(5):548-52. doi: 10.1111/j.1365-2036.2009.04207.x. Epub 2009 Nov 30.
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