抗菌活性、细胞毒性及白花羊角拗叶提取物（夹竹桃科）的植物化学筛选。
Antimicrobial activity, cytotoxicity, and phytochemical screening of Voacanga globosa (Blanco) Merr. leaf extract (Apocynaceae).
机构信息
Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines.
出版信息
Asian Pac J Trop Med. 2011 Oct;4(10):824-8. doi: 10.1016/S1995-7645(11)60202-2.
OBJECTIVE
To determine the antibacterial, antifungal, antiprotozoal, cytotoxic, and phytochemical properties of ethanol extracts of leaves of Voacanga globosa (Blanco) Merr. (V. globosa).
METHODS
The extracts were tested against bacteria and fungus through disc diffusion assay; against protozoa through growth curve determination, antiprotozoal and cytotoxicity assays.
RESULTS
The extract revealed antibacterial activities, inhibiting the growth of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, Micrococcus luteus, and Salmonella typhimurium. Antifungal assay showed that it inhibited Candida albicans. The antiprotozoal assay against Trichomonas vaginalis and Entamoeba histolytica showed that V. globosa can inhibit the parasites, wherein the action can be comparable to metronidazole. With the in situ cell death detection kit, Trichomonas vaginalis and Entamoeba histolytica exposed to V. globosa leaf extract was observed to fluoresce simultaneously in red and yellow signals signifying apoptotic-like changes. Preliminary phytochemical screening revealed the chemical composition of plant extract containing alkaloids, saponins, 2-deoxysugars, and hydrolysable tannins.
CONCLUSIONS
Thus, this study provides scientific evidence on the traditional use of V. globosa leaf extract in treating microbial diseases. Further, the leaf extract can possibly be used to produce alternative forms of antimicrobials.
目的
确定白花油麻藤（V. globosa）叶乙醇提取物的抗菌、抗真菌、抗原生动物、细胞毒性和植物化学特性。
方法
通过圆盘扩散法对提取物进行了抗菌和抗真菌测试；通过生长曲线测定、抗原生动物和细胞毒性测定来测试原生动物。
结果
该提取物显示出抗菌活性，抑制了金黄色葡萄球菌、蜡样芽孢杆菌、铜绿假单胞菌、藤黄微球菌和鼠伤寒沙门氏菌的生长。抗真菌试验表明它抑制了白色念珠菌。对阴道毛滴虫和溶组织内阿米巴的抗原生动物试验表明，白花油麻藤可以抑制寄生虫，其作用可与甲硝唑相媲美。使用原位细胞死亡检测试剂盒，观察到暴露于白花油麻藤叶提取物的阴道毛滴虫和溶组织内阿米巴同时发出红色和黄色信号，表明发生了类似凋亡的变化。初步的植物化学筛选揭示了植物提取物的化学成分，其中含有生物碱、皂苷、2-去氧糖和可水解单宁。
结论
因此，本研究为传统上使用白花油麻藤叶提取物治疗微生物疾病提供了科学依据。此外，该叶提取物可能可用于生产替代形式的抗菌药物。
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