（）乙醇叶提取物的植物化学筛选、抗菌及细胞毒性研究

PHYTOCHEMICAL SCREENING, ANTIMICROBIAL AND CYTOTOXICITY STUDIES OF ETHANOL LEAF EXTRACT OF ().

作者信息

Fall Alioune Dior, Bagla Victor Patrick, Bassene Emmanuel, Eloff Jacobus Nicolaas

机构信息

Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 Pretoria, South Africa.

Laboratory of Pharmacognosy and Botany; Faculty of Medicine, Pharmacy and Odontology. University Cheikh Anta DIOP, B.P 5005 Dakar-Fann, Senegal.

出版信息

Afr J Tradit Complement Altern Med. 2017 Jun 5;14(4):135-139. doi: 10.21010/ajtcam.v14i4.16. eCollection 2017.

DOI:10.21010/ajtcam.v14i4.16

PMID:28638876

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5471460/

Abstract

BACKGROUND

is commonly used in Senegalese traditional medicine to treat pain, inflammation, asthenia, bacterial and fungal infections. The aim of this study was to determine the type of phytochemical constituents present in the ethanol leaf extract and its antimicrobial activity against selected bacterial and fungal pathogens.

MATERIALS AND METHODS

The ethanol leaf extract of . was evaluated for its cytotoxic effect in the MTT assay against Vero cells. Flavonoids and tannins were the main constituents of the ethanol leaf extract.

RESULTS

The extract inhibited the growth of the three fungal strains used in this study moderately with the lowest MIC obtained for (0.16 mg/mL). The extract also inhibited the growth of and with an MIC of 0.62 mg/mL. For bacterial pathogens, strong inhibition was obtained against (ATTC 29212) (MIC 0.08 mg/mL), while moderate inhibition was obtained for (ATTC 25922) (MIC 0.16 mg/mL) and (ATTC 29213) (MIC 0.31mg/mL). The extract however did not inhibit the growth of (ATTC 27853) at the highest concentration (2.5 mg/ml) tested. The ethanol leaf extract of had a higher cytotoxicity than berberine used as the positive control (LC±0.04 µg/mL and 9.99±0.54 µg/mL respectively). The best selectivity index values was obtained for (SI = 1.24), followed by (SI = 0.62) for bacterial pathogens and (SI = 0.62) for fungal pathogens.

CONCLUSION

The findings of this study suggest that the extracts may not be safe for use in animals infected by some pathogens.

摘要

背景

在塞内加尔传统医学中常用于治疗疼痛、炎症、虚弱、细菌和真菌感染。本研究的目的是确定乙醇叶提取物中存在的植物化学成分类型及其对选定细菌和真菌病原体的抗菌活性。

材料与方法

对……的乙醇叶提取物进行了MTT法对Vero细胞的细胞毒性作用评估。黄酮类化合物和单宁是乙醇叶提取物的主要成分。

结果

该提取物对本研究中使用的三种真菌菌株的生长有中度抑制作用，对……获得的最低MIC为0.16mg/mL。该提取物还以0.62mg/mL的MIC抑制了……和……的生长。对于细菌病原体，对……（ATTC 29212）有强烈抑制作用（MIC 0.08mg/mL），而对……（ATTC 25922）（MIC 0.16mg/mL）和……（ATTC 29213）（MIC 0.31mg/mL）有中度抑制作用。然而，该提取物在测试的最高浓度（2.5mg/ml）下未抑制……（ATTC 27853）的生长。……的乙醇叶提取物比用作阳性对照的黄连素具有更高的细胞毒性（分别为LC±0.04μg/mL和9.99±0.54μg/mL）。对于细菌病原体，……获得了最佳的选择性指数值（SI = 1.24），其次是……（SI = 0.62），对于真菌病原体，……（SI = 0.62）。