一种多草药药物——什里沙迪的抗菌活性及支气管扩张作用评估。
Evaluation of antimicrobial activity and bronchodialator effect of a polyherbal drug-Shrishadi.
作者信息
Kajaria Divya Kumari, Gangwar Mayank, Kumar Dharmendra, Kumar Sharma Amit, Tilak Ragini, Nath Gopal, Tripathi Yamini Bhusan, Tripathi J S, Tiwari S K
机构信息
Faculty of Aurveda, Department of Kayachikitsa, Institute of Medical Sciences, Banaras Hindu University, Varanasi- 221005 (Uttar pradesh), India.
出版信息
Asian Pac J Trop Biomed. 2012 Nov;2(11):905-9. doi: 10.1016/S2221-1691(12)60251-2.
OBJECTIVE
To investigate antimicrobial and bronchodialator effect of hydroalcholic extract of polyherbal drug Shirishadi containing Shirisha (Albezzia lebbeck), Nagarmotha (Cyprus rotandus) & Kantakari (Solanum xanthocarpum).
METHODS
Antimicrobial activity was evaluated by disc diffusion method and MIC, MBC, MFC were calculated by micro dilution method. Hydroalcholic extract of this preparation was investigated for its phytochemical analysis, phenol and flavonoid were determined by spectrophotometric method and in vivo bronchodilator effect was analysed by convulsion time.
RESULTS
The phytochemical tests revealed presence of alkaloids, anthraquinones, carbohydrates, flavonoids, saponins and tannins. The antimicrobial result showed the MIC of 6.25 mg/mL against Staphylococcus aureus and 12.5 mg/mL for Escherichia coli and 12.5 mg/mL against remaining bacteria tested, with strong antifungal activity. The maximum inhibition zone is found against Pseudomonas aeruginosa with MIC 16 mg/mL. Drug showed significant bronchodilator effect with 27.86% & 36.13% increase in preconvulsion time of guinea pigs pretreated with 100 & 200 mg/kg body weight of extract.
CONCLUSIONS
The study reveals that the extracts possess antibacterial activity and antifungal activity in a dose dependent manner. This antimicrobial property may be due to presence of several saponins, further studies are highly needed for the drug development.
目的
研究含Shirisha（阔荚合欢）、Nagarmotha（圆叶刺蕊草）和Kantakari（刺天茄）的多草药药物Shirishadi水醇提取物的抗菌和支气管扩张作用。
方法
采用纸片扩散法评估抗菌活性，通过微量稀释法计算最低抑菌浓度（MIC）、最低杀菌浓度（MBC）和最低杀真菌浓度（MFC）。对该制剂的水醇提取物进行植物化学分析，采用分光光度法测定酚类和黄酮类化合物，并通过惊厥时间分析体内支气管扩张作用。
结果
植物化学测试显示存在生物碱、蒽醌、碳水化合物、黄酮类化合物、皂苷和单宁。抗菌结果表明，对金黄色葡萄球菌的MIC为6.25 mg/mL，对大肠杆菌为12.5 mg/mL，对其余测试细菌为12.5 mg/mL，具有较强的抗真菌活性。对铜绿假单胞菌的最大抑菌圈，MIC为16 mg/mL。该药物对豚鼠具有显著的支气管扩张作用，用100和200 mg/kg体重提取物预处理后，惊厥前时间分别增加27.86%和36.13%。
结论
该研究表明提取物具有剂量依赖性的抗菌和抗真菌活性。这种抗菌特性可能归因于多种皂苷的存在，药物开发急需进一步研究。
Zotero 插件