Von Blomberg M, Boerrigter G H, Scheper R J
Immunology. 1978 Aug;35(2):361-7.
The interference of two simultaneous skin test reactions of intermediate strength has been studied in the guinea-pig, using four different antigens, i.e. ovalbumin, horse cytochrome c, PPD and oxazolone. Skin test reactions were evaluated at 4, 24 and 48 h by measuring three parameters: increase in skin thickness, diameter of erythema and intensity of erythema. When an Arthus reaction was elicited simultaneously with a delayed hypersensitivity (DH) reaction, no effect on the DH reaction was observed. When two simultaneous DH reactions were elicited with different antigens, the risk of interference appeared to be rather small. When, however, the same antigen was used for both skin tests, suppression of at least one parameter of a DH-reaction was found in almost all experiments. Suppression of one skin test by another one could not be reduced by introducing a large distance between the two skin tests. As complete inhibition of either of the parameters never occurred, multiple skin testing may allow one to obtain a qualitative impression of the state of delayed hypersensitivity; when, however, reliable quantitative data are needed, the performance of more than one skin test at a time should be avoided.
在豚鼠身上研究了两种中等强度同时进行的皮肤试验反应的干扰情况，使用了四种不同的抗原，即卵清蛋白、马细胞色素c、结核菌素纯蛋白衍生物（PPD）和恶唑酮。通过测量三个参数在4小时、24小时和48小时对皮肤试验反应进行评估：皮肤厚度增加、红斑直径和红斑强度。当阿瑟斯反应与迟发型超敏反应（DH）同时引发时，未观察到对DH反应有影响。当用不同抗原同时引发两种DH反应时，干扰风险似乎相当小。然而，当两种皮肤试验都使用相同抗原时，在几乎所有实验中都发现至少一个DH反应参数受到抑制。通过在两种皮肤试验之间设置较大距离，无法减少一种皮肤试验对另一种皮肤试验的抑制。由于任何一个参数都从未完全被抑制，多次皮肤试验可能会让人对迟发型超敏反应状态获得定性印象；然而，当需要可靠的定量数据时，应避免一次进行多个皮肤试验。
