Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147 002, India.
Indian J Pharmacol. 2011 Sep;43(5):551-6. doi: 10.4103/0253-7613.84971.
The present study was designed to investigate the ameliorative potential and possible mechanism of hydroalcoholic extract of flowers of P. granatum in glycerol-induced acute renal failure (ARF) in rats.
The rats were subjected to rhabdomyolytic ARF by single intramuscular injection of hypertonic glycerol (50% v/v; 8 ml/kg) and the animals were sacrificed after 24 hours of glycerol injection. The plasma creatinine, blood urea nitrogen, creatinine clearance, and histopathological studies were performed to assess the degree of renal injury.
Pretreatment with hydroalcoholic extract of flowers of P. granatum (125 and 250 mg/kg p.o. twice daily for 3 days) significantly attenuated hypertonic glycerol-induced renal dysfunction in a dose-dependent manner. BADGE (Bisphenol-A-diglycidyl ether) (30 mg/kg), a peroxisome proliferator-activated receptor (PPAR)-γ antagonist, and N(omega)-nitro-l-arginine-methyl ester (L-NAME) (10, 20, and 40 mg/kg), nitric oxide synthase inhibitor, were employed to explore the mechanism of renoprotective effects of Punica granatum. Administration of BADGE (30 mg/kg) and L-NAME (40 mg/kg) abolished the beneficial effects of P. granatum in glycerol-induced renal dysfunction.
Hydroalcoholic extract of flowers of P. granatum has ameliorative potential in attenuating myoglobinuric renal failure and its renoprotective effects involve activation of PPAR-γ and nitric oxide-dependent signaling pathway.
本研究旨在探讨石榴花水醇提取物对甘油诱导的大鼠急性肾衰竭（ARF）的改善作用及其可能机制。
通过单次肌肉注射高渗甘油（50%v/v；8ml/kg）使大鼠发生横纹肌溶解性 ARF，甘油注射后 24 小时处死动物。进行血浆肌酐、血尿素氮、肌酐清除率和组织病理学研究，以评估肾脏损伤程度。
石榴花水醇提取物（125 和 250mg/kg，po，每日两次，连续 3 天）预处理可显著减轻甘油诱导的肾功能障碍，呈剂量依赖性。Bisphenol-A-diglycidyl ether（BADGE）（30mg/kg），一种过氧化物酶体增殖物激活受体（PPAR-γ）拮抗剂，和 N（omega）-nitro-l-arginine-methyl ester（L-NAME）（10、20 和 40mg/kg），一氧化氮合酶抑制剂，用于探讨石榴对肾脏保护作用的机制。给予 BADGE（30mg/kg）和 L-NAME（40mg/kg）可消除石榴在甘油诱导的肾功能障碍中的有益作用。
石榴花水醇提取物具有减轻肌红蛋白尿性肾衰竭的潜力，其肾脏保护作用涉及激活 PPAR-γ和一氧化氮依赖的信号通路。
