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从 SOMAmer 生物标志物发现到诊断和临床应用：基于 SOMAmer 的简化多重蛋白质组分析。

From SOMAmer-based biomarker discovery to diagnostic and clinical applications: a SOMAmer-based, streamlined multiplex proteomic assay.

机构信息

SomaLogic, Inc., Boulder, Colorado, United States of America.

出版信息

PLoS One. 2011;6(10):e26332. doi: 10.1371/journal.pone.0026332. Epub 2011 Oct 17.

DOI:10.1371/journal.pone.0026332
PMID:22022604
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3195687/
Abstract

Recently, we reported a SOMAmer-based, highly multiplexed assay for the purpose of biomarker identification. To enable seamless transition from highly multiplexed biomarker discovery assays to a format suitable and convenient for diagnostic and life-science applications, we developed a streamlined, plate-based version of the assay. The plate-based version of the assay is robust, sensitive (sub-picomolar), rapid, can be highly multiplexed (upwards of 60 analytes), and fully automated. We demonstrate that quantification by microarray-based hybridization, Luminex bead-based methods, and qPCR are each compatible with our platform, further expanding the breadth of proteomic applications for a wide user community.

摘要

最近，我们报道了一种基于 SOMAmer 的高度多重化分析方法，用于生物标志物的鉴定。为了实现从高度多重化的生物标志物发现分析方法到适合诊断和生命科学应用的格式的无缝过渡，我们开发了一种简化的、基于平板的分析方法。该基于平板的分析方法具有稳健、灵敏（亚皮摩尔）、快速、可高度多重化（超过 60 种分析物）和全自动等特点。我们证明，基于微阵列杂交、Luminex 珠方法和 qPCR 的定量方法都与我们的平台兼容，进一步扩大了广泛用户群体的蛋白质组学应用范围。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e17/3195687/f394e86d462d/pone.0026332.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e17/3195687/7842d72e7544/pone.0026332.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e17/3195687/fad33dd8d8cb/pone.0026332.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e17/3195687/dcb7c5d09cf7/pone.0026332.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e17/3195687/2c20063c6f50/pone.0026332.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e17/3195687/f394e86d462d/pone.0026332.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e17/3195687/7842d72e7544/pone.0026332.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e17/3195687/fad33dd8d8cb/pone.0026332.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e17/3195687/dcb7c5d09cf7/pone.0026332.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e17/3195687/2c20063c6f50/pone.0026332.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e17/3195687/f394e86d462d/pone.0026332.g005.jpg

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