AGAMOUS 通过招募 Polycomb 蛋白直接抑制 WUSCHEL 来终止拟南芥花茎干细胞的维持。
AGAMOUS terminates floral stem cell maintenance in Arabidopsis by directly repressing WUSCHEL through recruitment of Polycomb Group proteins.
机构信息
Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, California 92521, USA.
出版信息
Plant Cell. 2011 Oct;23(10):3654-70. doi: 10.1105/tpc.111.091538. Epub 2011 Oct 25.
Abstract
Floral stem cells produce a defined number of floral organs before ceasing to be maintained as stem cells. Therefore, floral stem cells offer an ideal model to study the temporal control of stem cell maintenance within a developmental context. AGAMOUS (AG), a MADS domain transcription factor essential for the termination of floral stem cell fate, has long been thought to repress the stem cell maintenance gene WUSCHEL (WUS) indirectly. Here, we uncover a role of Polycomb Group (PcG) genes in the temporally precise repression of WUS expression and termination of floral stem cell fate. We show that AG directly represses WUS expression by binding to the WUS locus and recruiting, directly or indirectly, PcG that methylates histone H3 Lys-27 at WUS. We also show that PcG acts downstream of AG and probably in parallel with the known AG target KNUCKLES to terminate floral stem cell fate. Our studies identify core components of the network governing the temporal program of floral stem cells.
摘要
花分生组织细胞在停止作为干细胞维持之前产生一定数量的花器官。因此，花分生组织细胞为研究发育背景下干细胞维持的时间控制提供了理想的模型。AGAMOUS（AG），一个对于花分生组织细胞命运终止至关重要的 MADS 结构域转录因子，长期以来被认为是通过间接抑制干细胞维持基因 WUSCHEL（WUS）来发挥作用的。在这里，我们揭示了多梳组（PcG）基因在 WUS 表达的时间精确抑制和花分生组织细胞命运终止中的作用。我们表明，AG 通过与 WUS 基因座结合并直接或间接地招募组蛋白 H3 Lys-27 甲基化 PcG 来直接抑制 WUS 的表达。我们还表明，PcG 作用于 AG 下游，并且可能与已知的 AG 靶标 KNUCKLES 平行作用以终止花分生组织细胞命运。我们的研究确定了调控花分生组织细胞时间程序的网络的核心组成部分。
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