Laboratory of Toxicology (LATOX), Department of Clinical and Toxicology, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
BMC Nephrol. 2011 Oct 26;12:59. doi: 10.1186/1471-2369-12-59.
We sought to investigate the relationships among the plasma levels of carotenoids, tocopherols, endogenous antioxidants, oxidative damage and lipid profiles and their possible effects on the cardiovascular risk associated with hemodialysis (HD) patients.
The study groups were divided into HD and healthy subjects. Plasma carotenoid, tocopherol and malondialdehyde (MDA) levels, as well as erythrocyte reduced glutathione (GSH), were measured by HPLC. Blood antioxidant enzymes, kidney function biomarkers and the lipid profiles were analyzed by spectrophotometric methods.
Plasma lycopene levels and blood glutathione peroxidase (GPx) activity were significantly decreased in HD patients compared with healthy subjects. Total cholesterol, low-density lipoprotein cholesterol (LDL-c), creatinine, urea, MDA, GSH, superoxide dismutase (SOD) and catalase (CAT) were significantly increased in HD (p < 0.05). Lycopene levels were correlated with MDA (r = -0.50; p < 0.01), LDL-c (r = -0.38; p = 0.01) levels, the LDL-c/HDL-c index (r = -0.33; p = 0.03) and GPx activity (r = 0.30; p = 0.03). Regression models showed that lycopene levels were correlated with LDL-c (β estimated = -31.59; p = 0.04), while gender was correlated with the TC/HDL-c index and triglycerides. Age did not present a correlation with the parameters evaluated. GPx activity was negatively correlated with MDA levels and with the LDL-c/HDL-c and CT/HDL-c indexes.
Lycopene may represent an additional factor that contributes to reduced lipid peroxidation and atherogenesis in hemodialysis patients.
我们旨在研究类胡萝卜素、生育酚、内源性抗氧化剂、氧化损伤和血脂谱的血浆水平之间的关系及其对血液透析（HD）患者心血管风险的可能影响。
研究组分为 HD 组和健康对照组。通过高效液相色谱法测量血浆类胡萝卜素、生育酚和丙二醛（MDA）水平以及红细胞还原型谷胱甘肽（GSH）。采用分光光度法分析血液抗氧化酶、肾功能生物标志物和血脂谱。
与健康对照组相比，HD 患者的血浆番茄红素水平和血液谷胱甘肽过氧化物酶（GPx）活性显著降低。总胆固醇、低密度脂蛋白胆固醇（LDL-c）、肌酐、尿素、MDA、GSH、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）在 HD 组中显著增加（p < 0.05）。番茄红素水平与 MDA（r = -0.50；p < 0.01）、LDL-c（r = -0.38；p = 0.01）水平、LDL-c/HDL-c 指数（r = -0.33；p = 0.03）和 GPx 活性（r = 0.30；p = 0.03）呈负相关。回归模型显示，番茄红素水平与 LDL-c 呈负相关（β 估计值 = -31.59；p = 0.04），而性别与 TC/HDL-c 指数和甘油三酯呈正相关。年龄与评估的参数无相关性。GPx 活性与 MDA 水平以及 LDL-c/HDL-c 和 CT/HDL-c 指数呈负相关。
番茄红素可能是血液透析患者脂质过氧化和动脉粥样硬化减少的另一个附加因素。
