Servicio de Hematología, Hospital Universitario Dr. José E. González, Avenida Madero y Gonzalitos S/N, Colonia Mitras Centro, Monterrey, Nuevo León, México.
Am J Clin Pathol. 2011 Nov;136(5):721-6. doi: 10.1309/AJCPFB6EXO7BJVLR.
The objective of the study was to evaluate the current standard practice of using volume and total nucleated cell (TNC) count for the selection of cord blood (CB) units for cryopreservation and further transplantation. Data on 794 CB units whose CD34+ cell content was determined by flow cytometry were analyzed by using a receiver operating characteristic (ROC) curve model to validate the performance of volume and TNC count for the selection of CB units with grafting purposes. The TNC count was the best parameter to identify CB units having 2 × 10(6) or more CD34+ cells, with an area under the ROC curve of 0.828 (95% confidence interval, 0.800-0.856; P < .01) and an efficiency of 75.4%. Combination of parameters (TNC/mononuclear cells [MNCs], efficiency 74.7%; TNC/volume, efficiency 68.9%; and volume/MNCs, efficiency 68.3%) did not lead to improvement in CB selection. All CB units having a TNC count of 8 × 10(8) or more had the required CD34+ cell dose for patients weighing 10 kg or less.
本研究旨在评估目前用于选择脐血（CB）单位进行冷冻保存和进一步移植的体积和总核细胞（TNC）计数的标准实践。通过使用接收器操作特征（ROC）曲线模型分析了 794 个 CB 单位的数据，这些单位的 CD34+细胞含量通过流式细胞术确定，以验证体积和 TNC 计数在选择具有移植物目的的 CB 单位方面的性能。TNC 计数是识别具有 2×10(6)或更多 CD34+细胞的 CB 单位的最佳参数，ROC 曲线下面积为 0.828（95%置信区间，0.800-0.856；P <.01），效率为 75.4%。参数组合（TNC/单核细胞[MNC]，效率 74.7%；TNC/体积，效率 68.9%；和体积/MNC，效率 68.3%）并没有导致 CB 选择的改善。所有 TNC 计数为 8×10(8)或更高的 CB 单位都具有 10 公斤或以下患者所需的 CD34+细胞剂量。
