Biomaterials and Nanomaterials Group, Faculty of Biology and Soil, Kazan (Idel buye/Volga region) Federal University, Kreml uramı 18, Kazan, Republic of Tatarstan, 420008, RF.
Langmuir. 2011 Dec 6;27(23):14386-93. doi: 10.1021/la203839v. Epub 2011 Nov 11.
Functionalized living cells are regarded as effective tools in directed cell delivery and tissue engineering. Here we report the facile functionalization of viable isolated HeLa cells with superparamagnetic cationic nanoparticles via a single-step biocompatible process. Nanoparticles are localized on the cellular membranes and do not penetrate into the cytoplasm. The magnetically responsive cells are viable and able to colonize and grow on substrates. Magnetically facilitated microorganization of functionalized cells into viable living clusters is demonstrated. We believe that the technique described here may find a number of potential applications in cell-based therapies and in development of whole-cell biosensors.
功能化的活细胞被认为是定向细胞输送和组织工程的有效工具。在这里，我们报告了通过一步生物相容的过程，将超顺磁性阳离子纳米粒子轻易地功能化到活的分离的 HeLa 细胞上。纳米粒子定位于细胞膜上，不会渗透到细胞质中。磁性响应的细胞是有活力的，能够在基质上定植和生长。证明了功能化细胞通过磁性辅助微组织成有活力的活细胞簇。我们相信，这里描述的技术可能在基于细胞的治疗和全细胞生物传感器的开发中找到许多潜在的应用。
