Steffen D G, Phinney G, Brown L J, Mersmann H J
J Lipid Res. 1979 Feb;20(2):246-53.
Enzymes associated with glycerolipid biosynthesis were examined in microsomal fractions of liver and adipose tissue obtained from swine of various ages. Generally, liver glycerophosphate acyltransferase, phosphatidate phosphohydrolase, diglyceride acyltransferase, and choline phosphotransferase activities were substantial at birth but increased 2- to 3-fold by day 14 postpartum, decreased at day 25, then increased at the oldest ages studied (up to 155 days postpartum). In adipose tissue, enzyme activities were low at birth and developed through day 25 in a pattern generally similar to that observed in liver. In contrast to liver, the adipose enzymes were depressed immediately postweaning (day 32) with subsequent recovery. The observed decline in adipose tissue enzyme activities expressed on a tissue basis at older ages was primarily the result of increased adipocyte size, since the activities expressed on a cell basis did not decline as rapidly. In both liver and adipose tissue, phosphatidate was the major glycerolipid synthesized by the microsomal glycerophosphate acyltransferase enzymes at all ages (generally greater than 75%). The ratio of neutral lipids to phospholipids produced by acylation of glycerophosphate was increased when a microsomal--cytosolic preparation was used as a source of enzyme in contrast to a microsomal preparation.
对从不同年龄段猪获取的肝脏和脂肪组织微粒体部分中与甘油脂质生物合成相关的酶进行了检测。一般来说，肝脏甘油磷酸酰基转移酶、磷脂酸磷酸水解酶、甘油二酯酰基转移酶和胆碱磷酸转移酶的活性在出生时就很高，但在产后14天时增加了2至3倍，在25天时下降，然后在研究的最大年龄（产后155天）时又增加。在脂肪组织中，酶活性在出生时较低，并在25天内以与肝脏中观察到大致相似的模式发展。与肝脏不同的是，脂肪酶在断奶后立即（第32天）下降，随后恢复。在较大年龄时，以组织为基础观察到的脂肪组织酶活性下降主要是脂肪细胞大小增加的结果，因为以细胞为基础的活性下降没有那么快。在肝脏和脂肪组织中，磷脂酸是所有年龄段微粒体甘油磷酸酰基转移酶合成的主要甘油脂质（一般大于75%）。与微粒体制剂相比，当使用微粒体 - 胞质制剂作为酶源时，由甘油磷酸酰化产生的中性脂质与磷脂的比例增加。
