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Proc Natl Acad Sci U S A. 2004 May 25;101(21):8156-61. doi: 10.1073/pnas.0402443101. Epub 2004 May 17.
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Novel internal controls for real-time PCR assays.实时聚合酶链反应检测的新型内部控制措施。
Clin Chem. 2004 May;50(5):801-2. doi: 10.1373/clinchem.2004.032672.
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Factors that make an infectious disease outbreak controllable.使传染病爆发可控的因素。
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Rapid detection of the severe acute respiratory syndrome (SARS) coronavirus by a loop-mediated isothermal amplification assay.通过环介导等温扩增法快速检测严重急性呼吸综合征(SARS)冠状病毒。
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J Clin Microbiol. 2004 Mar;42(3):1348-52. doi: 10.1128/JCM.42.3.1348-1352.2004.
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Avian influenza A (H5N1) in 10 patients in Vietnam.越南10例甲型H5N1禽流感患者
N Engl J Med. 2004 Mar 18;350(12):1179-88. doi: 10.1056/NEJMoa040419. Epub 2004 Feb 25.
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Use of loop-mediated isothermal amplification of the IS900 sequence for rapid detection of cultured Mycobacterium avium subsp. paratuberculosis.使用IS900序列的环介导等温扩增技术快速检测培养的副结核分枝杆菌亚种。
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通过环介导等温扩增法检测甲型人流感病毒

Detection of human influenza A viruses by loop-mediated isothermal amplification.

作者信息

Poon Leo L M, Leung Cynthia S W, Chan Kwok H, Lee Jack H C, Yuen Kwok Y, Guan Yi, Peiris Joseph S M

机构信息

Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR.

出版信息

J Clin Microbiol. 2005 Jan;43(1):427-30. doi: 10.1128/JCM.43.1.427-430.2005.

DOI:10.1128/JCM.43.1.427-430.2005
PMID:15635005
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC540134/
Abstract

Here we describe the use of the loop-mediated isothermal amplification (LAMP) method to detect human influenza viruses (H1 to H3). Our results were correlated 100% with results deduced from routine clinical diagnostic tests. In addition, we also developed a LAMP assay specific for human beta-actin cDNA as a quality control test.

摘要

在此,我们描述了使用环介导等温扩增(LAMP)方法检测人类流感病毒(H1至H3)。我们的结果与常规临床诊断测试得出的结果100%相关。此外,我们还开发了一种针对人类β-肌动蛋白cDNA的LAMP检测方法作为质量控制测试。