通过环介导等温扩增法检测甲型人流感病毒
Detection of human influenza A viruses by loop-mediated isothermal amplification.
作者信息
Poon Leo L M, Leung Cynthia S W, Chan Kwok H, Lee Jack H C, Yuen Kwok Y, Guan Yi, Peiris Joseph S M
机构信息
Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR.
出版信息
J Clin Microbiol. 2005 Jan;43(1):427-30. doi: 10.1128/JCM.43.1.427-430.2005.
Abstract
Here we describe the use of the loop-mediated isothermal amplification (LAMP) method to detect human influenza viruses (H1 to H3). Our results were correlated 100% with results deduced from routine clinical diagnostic tests. In addition, we also developed a LAMP assay specific for human beta-actin cDNA as a quality control test.
摘要
在此，我们描述了使用环介导等温扩增（LAMP）方法检测人类流感病毒（H1至H3）。我们的结果与常规临床诊断测试得出的结果100%相关。此外，我们还开发了一种针对人类β-肌动蛋白cDNA的LAMP检测方法作为质量控制测试。
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