Department of Cell and Molecular Biology, Faculty of Sciences, University of A Coruña, Campus A Zapateira s/n, 15071, A Coruña, Spain.
Cell Biol Toxicol. 2013 Jun;29(3):189-97. doi: 10.1007/s10565-013-9246-1. Epub 2013 May 9.
Okadaic acid (OA), produced by dinoflagellates during harmful algal blooms (HAB), belongs to the Diarrheic Shellfish Poisoning toxins that cause gastrointestinal symptoms in humans after consumption. In the present work, Ruditapes decussatus haemocytes were selected to evaluate the effect of OA on cell viability, enzymatic status and immune capacity through the measure by flow cytometry of apoptosis-cell death, non-specific esterase activity and phagocytosis. In order to compare different exposure conditions, two experiments were developed: in vitro exposure to OA and HAB simulation by feeding clams with the OA producer, Prorocentrum lima. Apoptosis was not OA dose-dependent and cell death increased in both assays. Phagocytosis of latex beads and esterase activity decreased in haemocytes incubated with OA. In contrast, esterases increased during the feeding with P. lima. Our results showed that OA and the simulated HAB caused damages on haemocyte functions and viability.
冈田酸（OA）是赤潮藻产生的一种物质，属于腹泻性贝类毒素，人在食用受其污染的贝类后会出现胃肠道症状。本研究选用菲律宾蛤仔（Ruditapes decussatus）血细胞，通过流式细胞术检测细胞凋亡/死亡、非特异性酯酶活性和吞噬作用，评估 OA 对细胞活力、酶活性和免疫能力的影响。为了比较不同的暴露条件，设计了两个实验：体外暴露于 OA 和通过投喂产生 OA 的藻类（Prorocentrum lima）来模拟赤潮。在这两种检测方法中，凋亡均与 OA 剂量无关，细胞死亡均增加。与对照组相比，与 OA 共孵育的血细胞对乳胶珠的吞噬作用和酯酶活性降低，而在用 P. lima 投喂时酯酶活性增加。我们的结果表明，OA 和模拟赤潮都会对血细胞的功能和活力造成损伤。
