Santi Adriana, Menezes Charlene, Duarte Marta Maria F, Leitemperger Jossiele, Lópes Thais, Loro Vania L
Departamento de Química, Centro de Ciências Naturais e Exatas, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, RS, Brazil.
Interdiscip Toxicol. 2011 Sep;4(3):149-53. doi: 10.2478/v10102-011-0023-9.
The aim of this study was to investigate the effect of clomazone herbicide on oxidative stress biomarkers and acetylcholinesterase activity in human erythrocytes in in vitro conditions. The activity of catalase (CAT), superoxide dismutase (SOD) and acetylcholinesterase (AChE), as well as the levels of thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) were measured in human erythrocytes exposed (in vitro) to clomazone at varying concentrations in the range of 0, 100, 250 and 500 µg/L for 1 h at 37 °C.TBARS levels were significantly higher in erythrocytes incubated with clomazone at 100, 250 and 500 µg/L. However, erythrocyte CAT and AChE activities were decreased at all concentrations tested. SOD activity was increased only at 100 µg/L of clomazone. GSH levels did not change with clomazone exposure. These results clearly showed clomazone to induce oxidative stress and AChE inhibition in human erythrocytes (in vitro). We, thus, suggest a possible role of ROS on toxicity mechanism induced by clomazone in humans.
本研究的目的是在体外条件下研究异恶草酮除草剂对人红细胞氧化应激生物标志物和乙酰胆碱酯酶活性的影响。在37℃下,将人红细胞(体外)暴露于浓度分别为0、100、250和500μg/L的异恶草酮中1小时,然后测定过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和乙酰胆碱酯酶(AChE)的活性,以及硫代巴比妥酸反应性物质(TBARS)和还原型谷胱甘肽(GSH)的水平。在与100、250和500μg/L异恶草酮孵育的红细胞中,TBARS水平显著升高。然而,在所有测试浓度下,红细胞CAT和AChE活性均降低。仅在100μg/L异恶草酮时SOD活性增加。GSH水平在异恶草酮暴露后未发生变化。这些结果清楚地表明异恶草酮在体外可诱导人红细胞氧化应激和AChE抑制。因此,我们认为活性氧可能在异恶草酮对人类的毒性机制中发挥作用。