Instituto de Agroquı́mica y Tecnologı́a de Alimentos (CSIC), Apartado de Correos 73, 46100 Burjassot, Valencia, Spain.
Meat Sci. 2004 Jun;67(2):211-7. doi: 10.1016/j.meatsci.2003.10.008.
The present work is focused on the use of the relative amounts of carnosine and anserine for detecting the presence of proteins of mammalian origin in feeds for ruminants. The methodology is cheap and simple and may serve for the rapid screening of feeds. Animal protein additions to feeds as low as 0.5% can be easily detected based on the cation exchange HPLC determination of the carnosine and/or anserine dipeptides. Furthermore, the molar ratio of these dipeptides was found to be characteristic of the animal species and could be used to discriminate between mammalian and non-mammalian species eventually added to feeds. Feeds having carnosine/anserine molar ratios higher than 0.3 were strongly suspected of containing banned mammalian proteins. Those suspicious feeds might be later confirmed by more specific techniques. The exact source of feeds containing mixtures of animal proteins from different species could not be identified by this method even though the method was able to detect its mammalian origin.
本工作集中于使用肌肽和鹅肌肽的相对含量来检测反刍动物饲料中是否存在哺乳动物来源的蛋白质。该方法廉价且简单，可用于饲料的快速筛选。基于阳离子交换 HPLC 测定肌肽和/或鹅肌肽二肽，可轻松检测到低至 0.5%的动物蛋白添加物。此外，发现这些二肽的摩尔比是动物物种的特征，可以用于区分最终添加到饲料中的哺乳动物和非哺乳动物物种。肌肽/鹅肌肽摩尔比高于 0.3 的饲料强烈怀疑含有违禁的哺乳动物蛋白质。这些可疑的饲料可能会通过更具体的技术进行进一步确认。即使该方法能够检测出其哺乳动物来源，也无法通过该方法确定含有来自不同物种的动物蛋白质混合物的饲料的确切来源。
