Laboratory of Animal Breeding and Genetics, Graduate School of Science and Technology, Kobe University, Kobe 657-8501, Japan.
Meat Sci. 2004 Jun;67(2):275-80. doi: 10.1016/j.meatsci.2003.10.016.
In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. This paper describes the development of DNA markers to discriminate between Japanese Black and F1 (Japanese Black×Holstein) breeds. Amplified fragment length polymorphism method was employed to detect candidate markers absent in Japanese Black but present in Holstein. The 500 primer combinations yielded six selected markers that were converted into single nucleotide polymorphisms markers for high-throughput genotyping. The allele frequencies in both breeds were investigated for discrimination ability using PCR-RFLP. The probability of identifying F1 was 0.882 and probability of misjudgment was 0.0198. The markers could be useful for discriminating between Japanese Black and F1 and would contribute to the elimination of falsified breed labeling of meat.
在肉类行业，食品标签上需要准确的品种信息，以确保肉品质量。遗传标记为品种鉴定提供了佐证证据。本文描述了开发 DNA 标记来区分日本黑牛和 F1（日本黑牛×荷斯坦牛）品种的方法。采用扩增片段长度多态性方法检测在日本黑牛中不存在但在荷斯坦牛中存在的候选标记。从 500 个引物组合中筛选出 6 个标记，将其转化为用于高通量基因分型的单核苷酸多态性标记。使用 PCR-RFLP 分析了两种品种的等位基因频率，以评估其鉴别能力。鉴定 F1 的概率为 0.882，误判的概率为 0.0198。这些标记可用于区分日本黑牛和 F1，有助于消除肉类伪造品种标签的问题。
