Department of Emergency Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA.
J Med Toxicol. 2011 Dec;7(4):288-94. doi: 10.1007/s13181-011-0189-8.
The advancement of molecular biologic techniques and their capabilities to answer questions pertaining to mechanisms of pathophysiologic events have greatly expanded over the past few years. In particular, these opportunities have provided researchers and clinicians alike the framework from with which to answer clinical questions not amenable for elucidation using previous, more antiquated methods. Utilizing extremely small molecules, namely microRNA, DNA, protein, and nanoparticles, we discuss the background and utility of these approaches to the progressive, practicing physician. Finally, we consider the application of these tools employed as future bedside point of care tests, aiding in the ultimate goal of unsurpassed patient care.
近年来，分子生物技术的进步及其解答病理生理事件机制相关问题的能力有了极大的扩展。特别是，这些机会为研究人员和临床医生提供了一个框架，使他们能够回答以前更陈旧的方法无法阐明的临床问题。我们利用极其微小的分子，即 microRNA、DNA、蛋白质和纳米颗粒，讨论了这些方法对不断发展的执业医师的背景和应用。最后，我们考虑了将这些工具作为未来床边即时护理测试应用的问题，以帮助实现卓越的患者护理这一最终目标。
