UPMC Univ Paris 06, ERL INSERM U1057/UMR7203, Faculty of Medicine Pierre et Marie Curie, CHU Saint-Antoine, 27 rue de Chaligny 75012 Paris, France.
Microbes Infect. 2012 Mar;14(3):273-8. doi: 10.1016/j.micinf.2011.10.007. Epub 2011 Oct 25.
Rotaviruses attach to intestinal cells in a process that requires glycan recognition. Some bacteria from the gut microflora have been shown to modify cell-surface glycans. In this study, human intestinal cultured cells were incubated with bacteria-derived soluble factors and infected with rotavirus. Results show that only bacterial soluble factors that increase cell-surface galactose namely, those of Bacteroides thetaiotaomicron and Lactobacillus casei were able to efficiently block rotavirus infections. Increasing cell-surface galactose using galactosyltransferase resulted in a similar blockage of rotavirus infections. These results indicate that manipulation of cell-surface intestinal glycans by bacterial soluble factors can prevent rotavirus infection in a species-specific manner, and should now be considered a potential therapeutic approach against rotavirus infection.
轮状病毒通过糖基识别过程附着在肠道细胞上。肠道微生物群中的一些细菌已被证明可以修饰细胞表面糖链。在这项研究中，用人肠培养细胞与细菌来源的可溶性因子孵育，然后用轮状病毒感染。结果表明，只有能增加细胞表面半乳糖的细菌可溶性因子（即双歧杆菌和干酪乳杆菌）才能有效地阻断轮状病毒感染。使用半乳糖基转移酶增加细胞表面半乳糖也会导致轮状病毒感染的类似阻断。这些结果表明，细菌可溶性因子对肠道表面糖链的操纵可以以种属特异性的方式防止轮状病毒感染，现在应该被视为一种针对轮状病毒感染的潜在治疗方法。
