Department of Physical Education, Anhui Defence Vacation College, Liouan, Anhui Province 237011, China.
Pharmacol Biochem Behav. 2012 Jan;100(3):554-9. doi: 10.1016/j.pbb.2011.10.026. Epub 2011 Nov 6.
Although galanin has been shown to increase insulin sensitivity in skeletal muscle of rats, there is no literature available about the effect of galanin on Glucose Transporter 4 (GLUT4) translocation from intracellular membrane pools to plasma membranes in adipocytes of type 2 diabetic rats. In the present study M35, a galanin antagonist was used to elucidate whether exercise-induced galanin release increased GLUT4 translocation in adipocytes of streptozotocin-induced diabetic rats. The present findings showed that plasma galanin levels after swimming training in all four trained groups were higher compared with each sedentary control. M35 treatment had an inhibitory effect on glucose infusion rates in the euglycemic-hyperinsulinemic clamp test and GLUT4 mRNA expression levels in adipocytes. Moreover, M35 treatment reduced GLUT4 concentration in both plasma membranes and total cell membranes. The ratios of GLUT4 contents in plasma membranes to total cell membranes in four drug groups were lower compared with each control. These data demonstrate a beneficial role of endogenous galanin to transfer GLUT4 from internal stores to plasma membranes in adipocytes of type 2 diabetic rats. Galanin plays a significant role in regulation of glucose metabolic homeostasis and is an important hormone relative to diabetes.
尽管甘丙肽已被证明可增加大鼠骨骼肌的胰岛素敏感性，但目前尚无关于甘丙肽对 2 型糖尿病大鼠脂肪细胞中葡萄糖转运蛋白 4（GLUT4）从细胞内膜池向质膜易位的影响的文献报道。在本研究中，使用甘丙肽拮抗剂 M35 来阐明游泳训练是否会增加链脲佐菌素诱导的糖尿病大鼠脂肪细胞中甘丙肽的释放，从而增加 GLUT4 易位。目前的研究结果表明，与所有久坐对照组相比，所有四个训练组在游泳训练后血浆甘丙肽水平均升高。M35 处理对在正常血糖高胰岛素钳夹试验中的葡萄糖输注率和脂肪细胞中 GLUT4 mRNA 表达水平有抑制作用。此外，M35 处理降低了质膜和总细胞膜中 GLUT4 的浓度。与对照组相比，四个药物组中质膜中 GLUT4 含量与总细胞膜中 GLUT4 含量的比值均降低。这些数据表明，内源性甘丙肽在 2 型糖尿病大鼠脂肪细胞中将 GLUT4 从内部储存库转移到质膜中具有有益作用。甘丙肽在调节葡萄糖代谢稳态方面起着重要作用，是与糖尿病相关的重要激素。
